Comparative studies between humans and golden Syrian hamsters via thromboelastography  

作　　者：Ze Yang Lili Xie Jingjing Ba Simin Zan Letong Zhang Xinyi Zhang Yang Yu 

机构地区：[1]School of Laboratory Animal&Shandong Laboratory Animal Center,Shandong First Medical University&Shandong Academy of Medical Sciences,Jinan,China [2]The Second Affiliated Hospital of Shandong First Medical University,Taian,China

出　　处：《Animal Models and Experimental Medicine》2024年第4期570-577,共8页动物模型与实验医学（英文）

基　　金：Student Research Training Program,Grant/Award Number:2022104391282;Shandong Natural Science Foundation,Grant/Award Number:ZR2019MH021;National Natural Science Foundation of China,Grant/Award Number:81970385。

摘　　要：Background:Thromboelastography(TEG)is a widely utilized clinical testing method for real-time monitoring of platelet function and the thrombosis process.Lipid metab-olism disorders are crucial risk factors for thrombosis.The lipid metabolism charac-teristics of hamsters resemble those of humans more closely than mice and rats,and their relatively large blood volume makes them suitable for studying the mechanisms of thrombosis related to plasma lipid mechanisms.Whole blood samples from golden Syrian hamsters and healthy humans were obtained following standard clinical pro-cedures.TEG was employed to evaluate coagulation factor function,fibrinogen(Fib)function,platelet function,and the fibrinolytic system.Methods:The whole blood from hamster or healthy human was isolated following the clinical procedure,and TEG was employed to evaluate the coagulation factor func-tion,Fib function,platelet function,and fibrinolytic system.Coagulation analysis used ACLTOP750 automatic coagulation analysis pipeline.Blood routine testing used XN-2000 automatic blood analyzer.Results:TEG parameters revealed that hamsters exhibited stronger coagulation fac-tor function than humans(reaction time[R],p=0.0117),with stronger Fib function(alpha angle,p<0.0001;K-time[K],p<0.0001).Platelet function did not differ signifi-cantly(maximum amplitude[MA],p=0.077).Hamsters displayed higher coagulation status than humans(coagulation index[CI],p=0.0023),and the rate of blood clot dissolution in hamsters differed from that in humans(percentage lysis 30 min after MA,p=0.02).Coagulation analysis parameters indicated that prothrombin time(PT)and activated partial thromboplastin time(APTT)were faster in hamsters than in hu-mans(PT,p=0.0014;APTT,p=0.03),whereas the Fib content was significantly lower in hamsters than in humans(p<0.0001).No significant difference was observed in thrombin time(p=0.1949).Conclusions:In summary,TEG could be used to evaluate thrombosis and bleeding parameters in whole blood samples from hamsters.The platelet function of hamsters

关 键 词：platelet function Syrian hamster thromboelastography(TEG) THROMBOSIS 

分 类 号：R-332[医药卫生]