微小膜壳绦虫对ICR小鼠肠道菌群的影响  

Effects of Hymenolepis nana on intestinal flora of ICR mice

作　　者：李小毛 赵敬 王鸿雁 张科 牟荣[1,2] LI Xiaomao;ZHAO Jing;WANG Hongyan;ZHANG Ke;MOU Rong(Department of Parasitology,School of Basic Medicine,Guizhou Medical University,Guiyang 550025,China;Characteristic and Key Laboratory of Modern Pathogenic Biology,Guizhou Medical University,Guiyang 550025,China)

机构地区：[1]贵州医科大学基础医学院人体寄生虫学教研室,贵阳550025 [2]贵州医科大学现代病原生物学特色重点实验室,贵阳550025

出　　处：《黑龙江畜牧兽医》2024年第17期8-12,108-110,共8页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(82160398);贵州省普通高等学校青年科技人才成长项目(黔教合KY字[2021]153)。

摘　　要：为了探究微小膜壳绦虫(Hymenolepis nana)对ICR小鼠肠道菌群的影响,试验采用随机分组方式将30只ICR小鼠分为感染组和对照组,每组15只,感染组灌胃感染0.2 mL鉴定和纯化后的微小膜壳绦虫虫卵配制成的虫卵悬浊液(浓度为2500个/mL),对照组灌胃等量生理盐水,并于感染后第2,4,6,8,10天采集空肠内容物,采用16S rDNA扩增子测序分析技术检测16S rDNA扩增产物,所得的数据使用多组间方差分析进行两两比较,对肠道菌群的种类、分布和丰度状况进行研究。结果表明:菌群种类繁多,以厚壁菌门(占比为64%)、拟杆菌门(占比为25%)为主,其次是变形菌门(占比为6%)和放线菌门(占比为3%)。感染微小膜壳绦虫后第4,6,8,10天肠道菌群α多样性Sobs值呈现先下降再上升的趋势,第8天显著低于第4,6,10天(P<0.05);感染组与对照组组间谱系多样性指数差异不显著(P>0.05);与对照组相比,感染组相对丰度上调的菌群主要从属厚壁菌门,有紫单胞菌科、毛螺菌科和乳杆菌科;下调的菌群种类较多,主要从属厚壁菌门、拟杆菌门、放线菌门和变形菌门。与对照组相比,感染后第10天感染组小鼠肠道乳杆菌属的相对丰度升高最明显;葡萄球菌属、咸海鲜球菌属和棒杆菌属的相对丰度降低。说明微小膜壳绦虫感染会导致宿主肠道菌群失调,其中乳杆菌属的相对丰度变化最明显。To investigate the effects of Hymenolepis nana on intestinal flora of ICR mice,thirty ICR mice were randomly divided into the infection group and the control group,with 15 mice in each group.The infection group was given 0.2 mL of egg suspension by gavage,and a total of 2500 eggs/mL were prepared from the identified and purified Hymenolepis minuta eggs.The control group was given gavage with the same amount of normal saline.Jejunum contents were collected on 2,4,6,8,and 10 days after infection.16S rDNA amplicon sequencing technology was used to detect 16S rDNA amplification products in intestinal contents,and the data obtained were panded by multi-group ANOVA to study the species distribution and abundance of intestinal flora.The results showed that there were many types of bacteria,which were mainly Firmicutes(64%)and Bacteroidetes(25%),followed by Proteobacteria(6%)and Actinobacteria(3%).The diversity of intestinal flora on day 4,6,8,and 10 after infection showed a trend of first decreasing and then increasing,and the diversity on day 8 was significantly lower than that on day 4,6,and 10(P<0.05).There was no significant difference in species evolution between the infection group and the control group(P>0.05).Compared with the control group,the up-regulated flora in the infection group mainly belonged to Firmicutes,including Purpuromonas,Spirillaceae and Lactobacillaceae,while the down-regulated flora types mainly belonged to Firmicutes,Bacteroidetes,Actinobacteria and Proteobacteria.Compared with the control group,the relative abundance of Lactobacillus intestinalis in the infected group increased most significantly on the 10th day after infection.The relative abundance of Staphylococcus,Brassicococcus and Corynebacterium decreased significantly.It indicated that the infection of Hymenococcus minuta led to the imbalance of intestinal flora in the host,of which the relative abundance change of Lactobacillus was the most obvious.

关 键 词：微小膜壳绦虫 ICR小鼠 肠道菌群 菌群失调 乳杆菌属 

分 类 号：S852.734[农业科学—基础兽医学]