基于非靶向代谢组学探究水牛唾液和血清中的发情标志物  

Exploring the Changes of Estrous Markers in Saliva and Serum of Buffalo Based on Untargeted Metabolomics

作　　者：余洲 杨柏高 李崇阳[2] 曹建华 牛一凡 覃广胜[3] 王旭光[1] 万鹏程[4] 梁淦 赵学明[2] 阿布力孜·吾斯曼[1] YU Zhou;YANG Baigao;LI Chongyang;CAO Jianhua;NIU Yifan;QIN Guangsheng;WANG Xuguang;WAN Pengcheng;LIANG Gan;ZHAO Xueming;ABULIZI·Wusiman(College of Animal Sciences,Xinjiang Agricultural University,Urumqi 830052,China;Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Buffalo Research Institute,Chinese Academy of Agricultural Sciences and Guangxi Zhuang Nationality Autonomous Region,Nanning 530001,China;Institute of Animal Sciences of Xinjiang Academy of Agricultural and Reclamation Sciences,Shihezi 832099,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830052 [2]中国农业科学院北京畜牧兽医研究所,北京100193 [3]中国农业科学院广西壮族自治区水牛研究所,南宁530001 [4]新疆农垦科学院畜牧兽医研究所,石河子832099

出　　处：《中国草食动物科学》2024年第5期11-23,共13页China Herbivore Science

基　　金：国家重点研发计划政府间重点专项(2022YFE0100200);国家自然科学基金国际合作项目(32161143032);农业农村部和财政部-现代农业产业技术体系(CARS-36);国家家养动物种质资源库;中国农业科学院科技创新工程(ASTIP-IAS06)。

摘　　要：在现代化规模养殖中,水牛发情不明显的问题日益突出。研究旨在利用高通量非靶向代谢组学分析不同发情阶段水牛唾液和血清中代谢物的差异。在相同的饲养管理条件下,选择15头尼里-拉菲水牛,每头注射氯前列醇4 mL,注射当天计为第0天。分别在水牛发情前期(第1天)、发情期(第3天)、发情后期(第6天)早上饲喂之前(08:00-11:00)采集唾液和血清,样品处理后采用非靶向代谢组学检测。结果显示,不同发情阶段水牛的唾液和血清中均存在差异代谢物,发情期/发情前期水牛唾液中共鉴定出30种显著差异代谢物,血清中共鉴定出85种显著差异代谢物;发情期/发情后期水牛唾液中共鉴定出8种显著差异代谢物,血清中共鉴定出66种显著差异代谢物。这些差异代谢物主要富集在胰高血糖素信号通路、磷酸戊糖途径、甘油酯代谢等通路上。唾液代谢物与血清代谢物之间存在相关性。唾液中的差异代谢物D-2-磷酸甘油酸、L-丙酰肉碱、白三烯d4和8-异前列腺素a1可能作为水牛发情生物标志物的关键代谢物。The problem of inconspicuous estrus in buffaloes is becoming increasingly important in modern livestock production.The aim of this study was to analyse differences in salivary and serum metabolites of buffaloes at different estrus stages using high-throughput untargeted metabolomics.Under the same feeding and management conditions,15 Nyeri Rafi buffaloes were selected,each was injected with 4 mL of chloroprostol,and the day of injection was counted as the 0th day.Saliva and serum samples were collected in the morning before feeding(08:00-11:00 am)during proes‐trus(day 1),estrus(day 3)and metestrus(day 6),and they were detected using non-targeted metabolomics.The results showed that different metabolites were present in the saliva and serum of buffaloes at different estrous stages.30 signifi‐cantly different metabolites were identified in saliva and 85 significantly different metabolites in serum of buffaloes in the estrus/proestrus;8 significantly different metabolites were identified in saliva and 66 significantly different metabolites in serum of buffaloes in the estrus/metestrus.These different metabolites were mainly enriched in glucagon signalling path‐way,pentose phosphate pathway and glycerolipid metabolism pathways.And there was a correlation between salivary me‐tabolites and serum metabolites.These salivary metabolites,including D-2-phosphoglyceric acid,L-propionylcarnitine,Leukotriene d4,and 8-iso-prostaglandin a1,may serve as key metabolites for the biomarkers of estrus in buffalo.

关 键 词：水牛 发情鉴定 血清 唾液 代谢组学 

分 类 号：S823.83[农业科学—畜牧学]