lncRNA NEAT1在鼻咽癌中的表达情况及其对鼻咽癌放疗敏感性的影响  

Expression of lncRNA NEAT1 in nasopharyngeal carcinoma and its influence on radiotherapy sensitivity of nasopharyngeal carcinoma

作　　者：莫柒艳 黄欢 张维明 韦志松 石丹丽 韩露 农思凯 韩宇源 林国享 MO Qiyan;HUANG Huan;ZHANG Weiming;WEI Zhisong;SHI Danli;HAN Lu;NONG Sikai;HAN Yuyuan;LIN Guoxiang(Department of Oncology,Wuming Hospital of Guangxi Medical University,Nanning 530199,Guangxi,China)

机构地区：[1]广西医科大学附属武鸣医院肿瘤科,广西南宁市530199

出　　处：《广西医学》2024年第8期1217-1225,共9页Guangxi Medical Journal

基　　金：广西自然科学基金(2020GXNSFAA297109);广西医科大学青年科学基金项目(GXMUYSF202354);南宁市武鸣区科学研究与技术开发计划项目(20210124);广西高校中青年教师科研基础能力提升项目(2022KY0075、2024KY0095)。

摘　　要：目的探讨长链非编码RNA(lncRNA)核旁斑富集转录本1(NEAT1)在鼻咽癌中的表达情况及其对鼻咽癌放疗敏感性的影响。方法(1)使用UALCAN数据库分析NEAT1在头颈部鳞状细胞癌中的表达情况及其与患者临床分期、预后的关系。(2)利用实时荧光定量PCR检测鼻咽癌组织及正常鼻咽黏膜上皮、人永生化鼻咽上皮细胞NP-69及多株鼻咽癌细胞(CNE-1、CNE-2、CNE-2R、C666-1、C666-1R)中NEAT1的表达情况。(3)使用RNA干扰技术构建NEAT1沉默的CNE-2R细胞[NEAT1-短发夹RNA(shRNA)组],并设立对照组(未转染的CNE-2R细胞)、NEAT1-NC组(转染对照序列的CNE-2R细胞)。给予不同放射剂量X线照射后,检测3组CNE-2R细胞的存活率、凋亡率、克隆形成率及放射生物学参数。结果(1)NEAT1在头颈部鳞状细胞癌组织中呈高表达,且表达水平随着头颈部鳞状细胞癌分期的增加呈升高趋势(P<0.05),但不同NEAT1表达水平患者的总生存率差异无统计学意义(P>0.05)。(2)相较于正常鼻咽黏膜上皮组织,鼻咽癌组织中NEAT1表达上调(P<0.05)。相较于NP-69细胞,NEAT1在鼻咽癌细胞中的表达上调(P<0.05),其中CNE-2R细胞中的表达量最高(P<0.05);放射抗拒性细胞CNE-2R、C666-1R中NEAT1的表达量分别高于放疗敏感性细胞CNE-2、C666-1(P<0.05)。(3)在接受不同剂量X射线照射后,与对照组及NEAT1-NC组比较,NEAT1-shRNA组CNE-2R细胞存活率、克隆形成率及放射生物学参数降低,凋亡率升高(P<0.05),但对照组与NEAT1-NC组上述指标差异无统计学意义(P>0.05)。结论lncRNA NEAT1在鼻咽癌组织和细胞中呈高表达,与肿瘤分期密切相关,沉默NEAT1的表达可增加鼻咽癌的放疗敏感性。Objective To explore the expression of long non⁃coding RNA(lncRNA)nuclear paraspeckle assembly transcript 1(NEAT1)in nasopharyngeal carcinoma and its influence on radiotherapy sensitivity of nasopharyngeal carcinoma.Methods(1)NEAT1 expression in head and neck squamous cell carcinoma and its relation with clinical stage and prognosis of patients were analyzed through using the UALCAN database.(2)The real⁃time fluorescent quantitative PCR was used to detect NEAT1 expression in nasopharyngeal cancerous tissues and normal nasopharyngeal mucosal epithelium,in human immortalized nasopharyngeal epithelial cells NP⁃69,and in multiple strains of nasopharyngeal carcinoma cells(CNE⁃1,CNE⁃2,CNE⁃2R,C666⁃1,and C666⁃1R).(3)The RNA interference technique was used to construct NEAT1⁃silenced CNE⁃2R cells(the NEAT1⁃shot hairpin RNA[shRNA]group),and control group(non⁃transfection CNE⁃2R cells),and NEAT1⁃NC group(CNE⁃2R cells transfected with the control sequence)were set.After X⁃ray irradiation with different radian doses,the survival rate,apoptosis rate,cloning formation rate,and radiobiological parameters of CNE⁃2R cells were detected in the 3 groups.Results(1)NEAT1 was highly expressed in tissues of head and neck squamous cell carcinoma,and its expression was elevated with the progression of stage of head and neck squamous cell carcinoma(P<0.05),but there was no statistically significant difference in the total survival rate between patients with different NEAT1 expressions(P>0.05).(2)Compared with tissues of normal nasopharyngeal mucosal epithelium,NEAT1 expression was up⁃regulated in nasopharyngeal cancerous tissues(P<0.05).Compared with NP⁃69 cells,NEAT1 expression was up⁃regulated in nasopharyngeal cancerous tissues(P<0.05),therein expression in CNE⁃2R cells was the highest(P<0.05).NEAT1 expressions in radioresistant cells CNE⁃2R and C666⁃1R were higher than those in radiotherapy sensitivity cells CNE⁃2 and C666⁃1,respectively(P<0.05).(3)After receiving X⁃ray irradiation wi

关 键 词：鼻咽癌 放疗敏感性 长链非编码RNA 核旁斑富集转录本1 RNA干扰技术 

分 类 号：R739.63[医药卫生—肿瘤]