The Applications of CRISPR Screening in Aging Research  

CRISPR筛选在衰老研究中的应用

作　　者：XU Jia-Xin JING Yao-Bin QU Jing LIU Guang-Hui 徐嘉鑫;荆耀彬;曲静;刘光慧(海南医科大学(海南省医学科学院)衰老与肿瘤国际研究中心,海口571199;膜生物学国家重点实验室,北京100101;干细胞与生殖生物学国家重点实验室,北京100101;器官再生与智造重点实验室,北京100101;北京干细胞与再生医学研究院,北京100101;中国衰老标志物研究联合体,北京100101)

机构地区：[1]International Center for Aging and Cancer,Hainan Academy of Medical Sciences,Hainan Medical University,Haikou 571199,Hainan China [2]State Key Laboratory of Membrane Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [3]State Key Laboratory of Stem Cell and Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [4]State Key Laboratory of Organ Regeneration and Reconstruction,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China [5]Beijing Institute for Stem Cell and Regenerative Medicine,Beijing 100101,China [6]Aging Biomarker Consortium,Beijing 100101,China

出　　处：《中国生物化学与分子生物学报》2024年第9期1186-1196,共11页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.81921006,92149301,92168201,82330044,32341001);国家重点研发计划项目(No.2020YFA0804000,2020YFA0112200);中国科学院稳定支持基础研究领域青年团队计划(No.YSBR-076)和新基石科学基金会“科学探索奖”(No.2021-1045)海南省科技厅重点研发项目(No.2023ICAC-YANFA);海南省科技厅科技平台建设项目(No.2023ICAC-YUNXING)资助。

摘　　要：The advancement of Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR)gene editing technology has revolutionized the comprehension of human genome,propelling molecular and cellular biology research into unexplored realms and accelerating progress in life sciences and medicine.CRISPR-based gene screening,recognized for its efficiency and practicality,is widely utilized across diverse biological fields.Aging is a multifaceted process governed by a myriad of genetic and epigenetic factors.Unraveling the genes regulating aging holds promise for understanding this intricate phenomenon and devising strategies for its assessment and intervention.This review provides a comprehensive overview of the progress in CRISPR screening and its applications in aging research,while also offering insights into future directions.CRISPR-based genetic-manipulation tools are positioned as indispensable instruments for mitigating aging and managing age-related diseases.CRISPR基因编辑技术的发展改变了我们对人类基因组的理解,推动分子和细胞生物学研究迈向新的领域,加速了生命科学和医学的进展。基于CRISPR的基因筛选工具凭借其高效和便捷的优势,广泛应用于多个生物学领域。衰老是一个受遗传和表观遗传等多种因素调控的复杂过程,解析衰老调控基因有助于理解衰老、开发衰老评估和干预策略。本文概述了CRISPR筛选工具的进展及其在衰老研究中的应用,并展望其发展前景。CRISPR相关基因操纵工具有望成为延缓衰老和干预衰老相关疾病的重要手段。

关 键 词：Clustered Regularly Interspaced Short Palindromic Repeats(CRISPR) genetic screening AGING 

分 类 号：Q255[生物学—细胞生物学]