HPLC-DAD波长切换法同时测定仙芎骨康颗粒中淫羊藿苷、朝藿定C、宝藿苷I、阿魏酸的含量  

Simultaneous Determination of Icarin,Epimedin C,Baohuoside I and Ferulic Aci From Xianxionggukang Particles by HPLC-DAD

作　　者：叶怡馨 商佳璐 汪轩 章建华[2] 尹华[1] YE Yi-xin;SHANG Jia-lu;WANG Xuan;ZHANG Jian-hua;YIN Hua(School of Pharmaceutical Sciences,Zhejiang Chinese Medical University,Hangzhou 311402,China;First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州311402 [2]浙江中医药大学附属第一医院,浙江杭州310006

出　　处：《时珍国医国药》2024年第8期1798-1800,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(81874340);浙江省科技厅科技计划项目(2014C33216)。

摘　　要：目的建立HPLC-DAD波长切换法同时测定仙芎骨康颗粒中淫羊藿苷、朝藿定C、宝藿苷I、阿魏酸的含量。方法采用多波长切换法,色谱柱:Zorbax Eclipse SB-C_(18),以乙腈-1%冰醋酸水为流动相梯度洗脱,流速1.0 mL·min^(-1),柱温30℃。结果仙芎骨康颗粒中淫羊藿苷等4成分的峰面积与进样量均呈良好的线性关系(r>0.999);平均加样回收率为98.9%~102.4%(RSD<3.0%,n=6)。3批样品中淫羊藿苷、朝藿定C、宝藿苷I、阿魏酸含量限度分别为3.852、3.919、0.3553、0.9358 mg·g^(-1)。结论该方法简便快捷,结果准确可靠,方法重复性好,可用于仙芎骨康颗粒的质量控制,为制定质量标准草案提供依据。Objective To establish the simultaneous determination of icarin,epimedin C,baohuoside I and ferulic acid from Xianxionggukang particles by HPLC-DAD.Methods A multi wavelength switching method was used,the chromatographic col-umn was Zorbax Eclipse SB-Cis,with acetonitrile-1%glacial acetic acid water as the mobile phase gradient elution,flow rate 1.0 mL·min^(-1),column temperature 30℃C.Results The peak areas of patchouli glycosides and other 4 components in Xianx-ionggukang particles show good linear relationship with the injection volume(r>0.999);the average spiked recoveries are 98.9%~102.4%(RSD<3.0%,n=6).The limits of content of icarin,epimedin C,baohuoside I,and ferulic acid in the 3 batches of samples are 3.852,3.919,0.3553,and 0.9358 mg·g^(-1),respectively.Conclusion The method is simple and rap-id,with accurate and reliable results and good method reproducibility,which can be used for the quality control of Xianxionggu-kang particles and provide a basis for the development of draft quality standards.

关 键 词：仙芎骨康颗粒 淫羊藿苷 朝藿定C 宝藿苷I 阿魏酸 HPLC含量测定 

分 类 号：R284.1[医药卫生—中药学]