基于羟基氧化钴纳米片类酶特性荧光法检测食品中的甲醛  

Detection of formaldehyde in food by fluorescence method based on the oxidase-like activity of cobalt oxyhydroxide nanosheets

作　　者：詹蓉蓉 康瑞瑶 黄心洳 萧卓楠 刘佳焱 莫芸迪 郭琳苑 邓浩[3] 贾宝珠 吴民富 罗林[1] 徐振林[1] ZHAN Rongrong;KANG Ruiyao;HUANG Xinru;XIAO Zhuonan;LIU Jiayan;MO Yundi;GUO Linyuan;DENG Hao;JIA Baozhu;WU Minfu;LUO Lin;XU Zhenlin(Guangdong Key Laboratory of Food Quality and Safety,College of Food,South China Agricultural University,Guangzhou 510642,China;College of Biological and Food Engineering,Guangdong University of Education,Guangzhou 510303,China;Key Laboratory of Tropical Fruit and Vegetable Cold-chain of Hainan Province,Institute of Agro-products Processing and Design,Hainan Academy of Agricultural Sciences,Haikou 570103,China;Department of Food Science,Foshan Polytechnic,Foshan 528137,China)

机构地区：[1]广东省食品质量安全重点实验室,华南农业大学食品学院,广州510642 [2]广东第二师范学院生物与食品工程学院,广州510303 [3]海南省农业科学院农产品加工设计研究所,海南省热带果蔬冷链研究重点实验室,海口570103 [4]佛山职业技术学院食品科学系,佛山528137

出　　处：《分析试验室》2024年第9期1289-1295,共7页Chinese Journal of Analysis Laboratory

基　　金：广东省教育厅普通高校青年创新人才项目(2022KQNCX055);华南农业大学大学生创新创业训练计划项目(S202210564098);海南省热带果蔬冷链研究重点实验室开放基金(HAAS2023PT0210);广东大学生科技创新培育专项资金“攀登计划”项目(pdjh2023b0386,pdjh2022b0382)资助。

摘　　要：具有类氧化酶特性的羟基氧化钴纳米片(CoOOH)可催化邻苯二胺(OPD)氧化成黄色荧光2,3-二氨基吩嗪(DAP),而甲醛可与OPD发生反应产生席夫碱抑制DAP的生成,使体系在570 nm处的荧光强度降低。基于此,建立了一种检测食品中甲醛的荧光分析法。最佳条件下,该方法对甲醛的定量检测范围为5.2~59.3μmol/L,检出限为1.4μmol/L。以啤酒和冷冻水产品为样品进行添加回收实验,回收率均在94.6%~109.5%之间。该方法有望用于检测食品中的甲醛。The cobalt oxyhydroxide nanosheets(CoOOH)with oxidase-like activity can efficiently catalyzes the oxidation of o-phenylenediamine(OPD)to the yellow fluorescent 2,3-diaminophenazine(DAP),whereas the Schiff base reaction between OPD and formaldehyde can hinder the CoOOH-catalyzed transformation of OPD to DAP.Based on this,a rapid and sensitive fluorescence assay for the detection of formaldehyde in food was developed.Under the optimal conditions,the quantitative range of formaldehyde was 5.2-59.3μmol/L,and the limit of detection(LOD)was 1.4μmol/L.The recovery rates of beer and frozen aquatic products were all in the range of 94.6%-109.5%,indicating that the method can be used for the detection of formaldehyde in food.

关 键 词：甲醛 羟基氧化钴纳米片 荧光分析方法 类氧化物酶活性 

分 类 号：O657.31[理学—分析化学] X832[理学—化学]