竹叶黄酮对敌草快应激大鼠肝脏损伤、抗氧化功能及相关基因表达的影响  

Effects of bamboo leaf flavonoids on liver injury,antioxidant function and related gene expression in rats induced by diquat

作　　者：吴超 卢姝婉 史雪艳 杨彩梅 曾新福 张瑞强 刘金松 WU Chao;LU Shuwan;SHI Xueyan;YANG Caimei;ZENG Xinfu;ZHANG Ruiqiang;LIU Jinsong(College of Animal Science and Technology/College of Veterinary Medicine/Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province/Zhejiang Provincial Engineering Laboratory for Animal Health Inspection&Internet Technology,Zhejiang Agricultural and Forestry University,Hangzhou 311300,China;Zhejiang Vegamax Biotechnology Co.,Ltd.,Vegamax Green Animal Health Products Key Agricultural Enterprise Research Institute,Anji,Zhejiang313300,China)

机构地区：[1]浙江农林大学动物科技学院/动物医学院/浙江省畜禽绿色生态健康养殖应用技术研究重点实验室/动物健康互联网检测技术浙江省工程实验室,浙江杭州311300 [2]浙江省绿色动物保健品重点农业企业研究院浙江惠嘉生物科技股份有限公司,浙江安吉313300

出　　处：《中国兽医学报》2024年第7期1498-1506,共9页Chinese Journal of Veterinary Science

基　　金：浙江省尖兵领雁计划资助项目(2022C02059);浙江省领军型创新创业团队资助项目(2020R01015);杭州市农业和社会发展一般资助项目(202203B18);浙江省重点农业企业研究院资助项目(2021Y30004)。

摘　　要：旨在以大鼠为模型,研究竹叶黄酮(Bamboo leaf flavonoids,BLF)对敌草快(Diquat,DQ)应激动物肝脏损伤、抗氧化功能及相关基因表达的影响。选取32只5周龄雄性Sprague-Dawley(SD)大鼠,将其随机分为4组:对照组(Con)、1000mg/kg竹叶黄酮添加组(BLF)、DQ应激组(DQ)、1000mg/kg竹叶黄酮+DQ应激组(BLF-DQ)。结果显示,与Con组相比,DQ组大鼠血清谷草转氨酶(AST)的含量显著降低(P<0.05),肝脏总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GPX)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)水平显著减少(P<0.05),丙二醛(MDA)含量显著升高(P<0.05)。同时,肝脏血红素氧合酶-1(HO-1)、GPX、CAT、SOD1和核因子E2相关因子(Nrf2)的基因表达量显著降低(P<0.05);灌胃1000mg/kg BLF组大鼠血清AST和谷丙转氨酶(ALT)含量显著降低(P<0.05),肝脏T-AOC、GPX、CAT和SOD水平显著增强(P<0.05),肝脏HO-1、GPX、CAT、SOD1、Nrf2和醌氧化还原酶1(NQO1)基因表达水平显著上调(P<0.05);与DQ组相比,灌胃1000mg/kg BLF大鼠肝脏指数显著降低(P<0.05),血清中AST和ALT含量显著减少(P<0.05),同时肝脏中CAT、GPX、T-AOC水平显著增加(P<0.05),MDA含量显著降低(P<0.05),肝脏HO-1、GPX、CAT、SOD1和Nrf2的基因表达量显著上调(P<0.05)。结果表明,BLF能够缓解DQ应激引起的大鼠肝脏损伤,改善肝脏抗氧化功能抑制状态,激活Nrf2信号通路和PINK/Parkin线粒体自噬相关基因表达。Bamboo leaf flavonoids(BLF)are compounds extracted from bamboo leaves,possessing properties including antioxidant,antimicrobial and anti-inflammatory properties.This study aimed to investigate the effects of BLF on liver damage,antioxidant function,and related gene expression in rats induced by diquat(DQ).Thirty-two 5-week-old male Sprague-Dawley(SD)rats were randomly divided into four experimental groups:the control group(Con),1000mg/kg BLF group(BLF),DQ stress group(DQ),and 1000mg/kg BLF+DQ stress group(BLF-DQ).The results showed that compared to the Con,the DQ group exhibited significantly decreased serum AST levels(P<0.05),as well as decreased levels of T-AOC,GPX,SOD,and CAT in the liver(P<0.05),and increased MDA levels in rats(P<0.05).Additionally,the gene expression levels of HO-1,GPX,CAT,SOD1,and Nrf2in the liver were significantly reduced(P<0.05).In contrast,1000 mg/kg BLF significantly decreased serum AST and ALT levels(P<0.05),increased levels of TAOC,GPX,CAT,and SOD in liver(P<0.05),and significantly increased gene expression of HO-1,GPX,CAT,SOD1,Nrf2,and NQO1(P<0.05).Compared to the DQ group,BLF-DQ significantly decreased liver index(P<0.05),reduced serum AST and ALT levels(P<0.05),increased levels of CAT,GPX,and T-AOC in liver(P<0.05),decreased MDA levels(P<0.05),and significantly upregulated gene expression levels of HO-1,GPX,CAT,SOD1,and Nrf2(P<0.05).These findings indicated that BLF alleviate liver damage caused by DQ stress in rats,improve liver antioxidant function inhibition,activate the Nrf2signaling pathway and PINK/Parkin mitophagy-related gene expression.

关 键 词：大鼠 肝脏损伤 竹叶黄酮 抗氧化功能 敌草快 

分 类 号：S853.76[农业科学—临床兽医学]