福鼎白牡丹茶对变应性鼻炎大鼠Th1/Th2平衡及JAK2/STAT6信号通路的影响  

Effect of Fuding White Peony Tea on Th1/Th2 Balance and JAK2/STAT6 Signaling Pathway in Rats with Allergic Rhinitis

作　　者：曹洋 董博[2] 申力 李冬冬 苗青[3] 林小红 CAO Yang;DONG Bo;SHEN Li;LI Dongdong;MIAO Qing;LIN Xiaohong(Shaanxi University of Traditional Chinese Medicine,Xianyang Shaanxi,China 712046;Honghui Hospital Affiliated to Xi′an Jiaotong University,Xi′an Shaanxi China 710054;Institute of Basic Theory of Traditional Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing China 100700;Wupu Tea Industry Co.,Ltd.Of Fuding City,Fuding Fujian China 355200)

机构地区：[1]陕西中医药大学,陕西咸阳712046 [2]西安交通大学附属红会医院,陕西西安710054 [3]中国中医科学院中医基础理论研究所,北京100700 [4]福鼎市吾蒲茶业有限公司,福建福鼎355200

出　　处：《中医学报》2024年第10期2203-2209,共7页Acta Chinese Medicine

基　　金：国家自然科学基金面上项目(82074398);中国中医科学院科技创新工程项目(CI2021A03809)。

摘　　要：探讨福鼎白牡丹茶对变应性鼻炎(allergic rhinitis,AR)大鼠Th1/Th2细胞因子平衡及蛋白酪氨酸激酶2(Janus kinase 2,JAK2)/信号转导和转录激活因子6(signal transducer and activator of transcription 6,STAT6)信号通路的影响。方法:从50只SD雄性大鼠中随机选取10只作为正常组,其余大鼠采用卵清白蛋白致敏法建立AR模型。将造模成功的大鼠随机分为模型组、氯雷他定组(0.9 mg·kg^(-1))、白茶低剂量组(0.9 g·kg^(-1))及高剂量组(1.8 g·kg^(-1)),连续灌胃28 d。末次给药后大鼠进行行为学评分,采用ELISA法检测血清免疫球蛋白E(immunoglobulin E,IgE)、组胺(histamine,HIS)、干扰素-γ(interferon-γ,IFN-γ)、白细胞介素(interleukin)-4(IL-4)、IL-2、IL-5含量,HE染色观察鼻黏膜组织病理形态,Western blot和PCR法检测鼻黏膜T-bet、GATA-3、JAK2、STAT4、STAT6表达水平。结果:模型组大鼠的行为学评分较正常组升高(P<0.01),经氯雷他定、白茶治疗后降低(P<0.01)。与正常组比较,模型组大鼠血清IgE、HIS、IL-4、IL-5含量升高(P<0.01),IFN-γ、IL-2含量降低(P<0.01);经氯雷他定、白茶治疗后,上述因子的表达水平得到逆转(P<0.05)。HE染色显示,正常组大鼠鼻黏膜上皮结构未见明显异常;模型组大鼠鼻黏膜结构紊乱,上皮不完整,伴炎症细胞浸润;白茶治疗组大鼠鼻黏膜病理损伤较模型组缓解。Western blot和PCR显示,与正常组比较,模型组大鼠鼻黏膜GATA-3、JAK2、STAT6水平升高(P<0.01),T-bet、STAT4水平降低(P<0.01);经白茶高剂量治疗后,上述指标的表达水平得到逆转(P<0.05)。结论:福鼎白牡丹茶可能通过调节Th1/Th2细胞因子平衡及抑制JAK2/STAT6信号通路,有效改善AR大鼠症状。To investigate the effects of Fuding White Peony Tea on Th1/Th2 cytokine balance and protein tyrosine kinase 2(JAK2)/signal transducer and activator of transcription 6(STAT6)signaling pathway in rats with allergic rhinitis(AR).Methods:10 SD male rats were randomly selected as the normal group,and the rest of the rats were established by ovalbumin sensitization method.The rats were randomly divided into model group,loratadine group(0.9 mg·kg^(-1)),Fuding White Peony Tea low-dose group(0.9 g·kg^(-1))and high-dose group(1.8 g·kg^(-1))group and were continuously gavaged for 28 days.After the last dose,the rats were subjected to behavioral scores,and the contents of serum immunoglobulin E(IgE),histamine(HIS),interferon-γ(IFN-γ),interleukin-4(IL-4),IL-2 and IL-5 were detected by ELISA,and the histopathological morphology of nasal mucosa was observed by HE staining The expression levels of T-bet,GATA-3,JAK2,STAT4 and STAT6 in nasal mucosa were detected by blot and PCR.Results:The behavioral score of the model group was higher than that of the normal group(P<0.01)and decreased significantly after loratadine and white tea treatment(P<0.01).Compared with the normal group,the serum levels of IgE,HIS,IL-4 and IL-5 in the model group increased significantly(P<0.01),and the contents of IFN-γand IL-2 decreased significantly(P<0.01),and the expression levels of the above factors were reversed after treatment with loratadine and white tea(P<0.05).HE staining showed that there was no obvious abnormality in the nasal mucosal epithelial structure of the normal group.In the model group,the nasal mucosa structure was disordered,the epithelium was incomplete,and inflammatory cells were infiltrated.The pathological damage of nasal mucosa in the white tea treatment group was alleviated compared with that in the model group.Western blot and PCR showed that compared with the normal group,the levels of GATA-3,JAK2 and STAT6 in the nasal mucosa of the model group increased significantly(P<0.01),and the levels of T-bet and STAT4 decreas

关 键 词：福鼎白牡丹茶 白茶 变应性鼻炎 TH1/TH2平衡 JAK2/STAT6信号通路 免疫调节 大鼠 

分 类 号：R285.5[医药卫生—中药学]