β-石竹烯抑制LPS刺激巨噬细胞炎症的研究  

Study on the inhibition of β‑caryophyllene in lipopolysaccharide (LPS) induced inflammatory on macrophage

作　　者：杨丙晔 张燕琳 许依红 戴苑花 YANG Bingye;ZHANG Yanlin;XU Yihong;DAI Yuanhua(School of Pharmacy and Pharmaceutical Sciences,Xiamen Medical College,Xiamen 361023,Fujian,China;Xiamen Key Laboratory of Marine Medicinal Natural Products Resources,Xiamen 361023,Fujian,China)

机构地区：[1]厦门医学院药学系,福建厦门361023 [2]厦门市海洋药用天然产物资源重点实验室,福建厦门361023

出　　处：《生物资源》2024年第4期390-396,共7页Biotic Resources

基　　金：厦门市科技局医疗卫生指导项目(3502Z20214ZD1328);大学生创新创业训练项目(S202212631020);福建省教育厅中青年项目(JAT210478);厦门市海洋与渔业局青年科技创新项目(23YYZP004QCB28)。

摘　　要：β-石竹烯是一种双环倍半萜类化合物。为研究β-石竹烯在体外对脂多糖诱导炎症的作用效果及作用机制,利用β-石竹烯处理被脂多糖诱导的RAW264.7细胞炎症模型,用MTT法检测β-石竹烯对细胞活力的影响,用ELISA试剂盒检测IL-6、TNF-α炎症因子的含量变化,用q RT-PCR技术检测IL-6、TNF-α、IL-1β、MCP-1因子的m RNA含量变化,用Western blot检测NF-κB信号通路和JNK信号通路关键蛋白表达变化。结果显示,β-石竹烯能够降低细胞中IL-6因子的含量和IL-6、TNF-α、IL-1β、MCP-1因子的表达,抑制炎症信号通路NF-κB和JNK。因此,β-石竹烯可以通过抑制NF-κB和JNK信号通路,降低炎症因子的释放起到抗炎的效果。该研究为β-石竹烯活性的开发提供数据支持,并进一步为β-石竹烯应用于抗炎治疗方面提供了理论依据。β⁃caryophyllene is a dicyclic sesquiterpenoid compound.To study the anti⁃inflammatory effects and mech⁃anism ofβ⁃caryophyllene in lipopolysaccharide(LPS)-induced inflammatory,β⁃caryophyllene was used to treat RAW264.7 cell inflammatory model induced by LPS.The effects ofβ⁃caryophyllene on cell viability were detected by MTT assay,and the contents of IL⁃6 and TNF⁃αinflammatory factors were detected by ELISA kit.The mRNA levels of IL⁃6,TNF⁃α,IL⁃1βand MCP⁃1 were detected by qRT⁃PCR,and the proteins of NF⁃κB and JNK signaling pathways were detected by Western blot.The results show thatβ⁃caryophyllene can decrease the contents of IL⁃6 and the mRNA levels of IL⁃6,TNF⁃α,IL⁃1βand MCP⁃1,and decrease the protein contents of P65 and PJNK in RAW264.7 cells.Overall,these results suggest thatβ⁃caryophyllene has the anti⁃inflammatory effects by decreasing the secretion of inflam⁃matory cytokines Il⁃6,TNF⁃α,IL⁃1βand MCP⁃1 and inhibiting NF⁃κB and JNK signaling pathways.It provides the theoretical basis for developing the functional activity ofβ⁃caryophyllene,and furtherly provides a reference for the use ofβ⁃caryophyllene in the treatment of inflammation⁃related diseases.

关 键 词：β-石竹烯 炎症 小鼠腹腔巨噬细胞(RAW264.7) 炎症因子 信号通路 

分 类 号：R364.5[医药卫生—病理学]