Enhancer of Shoot Regeneration 2(ESR2)regulates pollen maturation and vitality in watermelon(Citrullus lanatus)  

作　　者：Hu Wang Lihong Cao Yalu Guo Zheng Li Huanhuan Niu 

机构地区：[1]College of Horticulture,Henan Agricultural University,Zhengzhou 450002,China [2]State Key Laboratory of Crop Stress Biology in Arid Areas,College of Horticulture,Northwest A&F University,Yangling 712100,China

出　　处：《Journal of Integrative Agriculture》2024年第10期3506-3521,共16页农业科学学报（英文版）

基　　金：support from the National Key Research and Development Program of China(2022YFD1602000);the National Natural Science Foundation of China(32202514,U22A20498 and 32072596);the Joint Fund of Henan Province Science and Technology Research and Development Plan,China(222103810009);the Science and Technology Innovation Team of Shaanxi,China(2021TD-32);the China Postdoctoral Science Foundation(2022M711064 and 2023M741062).

摘　　要：Watermelon(Citrullus lanatus)holds global significance as a fruit with high economic and nutritional value.Exploring the regulatory network of watermelon male reproductive development is crucial for developing male sterile materials and facilitating cross-breeding.Despite its importance,there is a lack of research on the regulation mechanism of male reproductive development in watermelon.In this study,we identified that ClESR2,a VIIIb subclass member in the APETALA2/Ethylene Responsive Factor(AP2/ERF)superfamily,was a key factor in pollen development.RNA insitu hybridization confirmed significantClESR2 expression in the tapetum and pollen during the later stage of anther development.The pollens of transgenic plants showed major defects in morphology and vitality at the late development stage.The RNA-seq and protein interaction assay confirmed that ClESR2 regulates pollen morphology and fertility by interacting with key genes involved in pollen development at both transcriptional and protein levels.These suggest that Enhancer of Shoot Regeneration 2(ESR2)plays an important role in pollen maturation and vitality.This study helps understand the male reproductive development of watermelon,providing a theoretical foundation for developing male sterile materials.

关 键 词：ESR2 WATERMELON OVEREXPRESSION pollen maturation pollen vitality 

分 类 号：S651[农业科学—果树学]