黏细菌A96-1产埃博霉素B的鉴定及其发酵培养基优化  

Identification of epothilone B produced by Myxobacterium A96-1 and optimization of fermentation medium

作　　者：翟辰欣 苏千景 刘言 柳远哲 葛燕 王泽建[3] 梁剑光 Zhai Chenxin;Su Qianjing;Liu Yan;Liu Yuanzhe;Ge Yan;Wang Zejian;and Liang Jianguang(School of Pharmacy&School of Biological and Food Engineering,Changzhou University,Changzhou 213164;Zhejiang Jinliyuan Pharmaceutical Limited Company,Shaoxing 312369;School of Biotechnology,East China University of Science and Technology,Shanghai 200237)

机构地区：[1]常州大学药学院生物与食品工程学院,常州213164 [2]浙江金立源药业有限公司,绍兴312369 [3]华东理工大学生物工程学院,上海200237

出　　处：《中国抗生素杂志》2024年第8期876-882,共7页Chinese Journal of Antibiotics

基　　金：江苏省研究生科研与实践创新计划项目(No.SJCX23_1456)。

摘　　要：目的 筛选埃博霉素B产生菌,并优化发酵培养基,以提高发酵产量。方法 利用高效液相色谱法和薄层色谱法对次级代谢产物进行分析鉴定,从实验室保存的菌库中筛选出埃博霉素B产生菌,再通过单因素实验和正交试验优化发酵培养基成分。结果 获得1株产埃博霉素B的黏细菌A96-1,采用优化后的发酵培养基:马铃薯淀粉6 g/L,玉米浆干粉2 g/L,CaCl_22 g/L,MgSO_4·7H_2O 3 g/L,微量元素溶液1 mL/L,树脂添加量2%,埃博霉素B发酵产量为251.4 mg/L,较优化前提高了3.83倍。结论通过菌株筛选和优化的培养基大幅提高了埃博霉素B的产量,为今后其资源开发利用及产业化提供参考。Objective To improve the production of epothilone B by screening of epothilone producing strains and optimizing the fermentation medium.Methods The secondary metabolites were identified by HPLC and TLC,and the epothilone B producing bacteria were screened out from the culture collection in our laboratory.The fermentation medium components were optimized by a single factor experiment and an orthogonal experiment.Results A Myxobacterium A96-1 producing epothilone B was obtained.The optimized fermentation medium consisted of potato starch(6 g/L),corn slurry dry powder(2 g/L),CaCl2(2 g/L),MgSO4·7H2O(3 g/L),trace element solution(1 mL/L),resin addition(2%).Under the conditions,the fermentation yield of this strain of epothilone B was 251.4 mg/L,which was 3.83 times higher than before optimization.Conclusion The production of epothilone B was significantly increased through strain screening and optimized medium,which provided a reference for the future resource development and utilization and industrialization of epothilone B.

关 键 词：黏细菌 埃博霉素B 发酵优化 薄层层析色谱法 正交实验 

分 类 号：R978.1[医药卫生—药品]