桑叶多酚的降糖作用机制研究  

Study on the Mechanism of Hypoglycemic Effect of Mulberry Leaf Polyphenols

作　　者：占鹏飞 陈田飞 赵辉 董馨恬 冯佳怡 柳秦桥 钟石[5] 孙雨晴[5] Zhan Pengfei;Chen Tianfei;Zhao Hui;Dong Xintian;Feng Jiayi;Liu Qinqiao;Zhong Shi;Sun Yuqing(Huzhou Academy of Agricultural Sciences,Huzhou Zhejiang 313000,China;Chun’an Agricultural and Rural Deve-lopment Service Center,Hangzhou 311700,China;College of Life Sciences,China Jiliang University,Hangzhou 310018,China;Huzhou Liuyin Biotechnology Co.,Ltd.,Huzhou Zhejiang 313009,China;Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]湖州市农业科学研究院,浙江湖州313000 [2]淳安县农业农村发展服务中心,杭州311700 [3]中国计量大学生命科学学院,杭州310018 [4]湖州柳荫生物科技有限公司,浙江湖州313009 [5]浙江省农业科学院,杭州310021

出　　处：《蚕业科学》2024年第3期242-251,共10页ACTA SERICOLOGICA SINICA

基　　金：湖州市现代农业技术研发项目(2022SY001);湖州市重点研发计划项目(2023ZD2028);浙江省农业重大技术协同推广计划试点项目(2024ZDXT08);淳安县农业科技项目。

摘　　要：桑叶自古以来就被用于治疗消渴症(糖尿病),现代医学研究也证明桑叶具有降糖功效。提取纯化获得桑叶多酚,通过蔗糖酶抑制试验、自由基清除试验、高糖诱导HepG2肝细胞胰岛素抵抗试验等多种手段,研究桑叶多酚降糖作用的机制。结果显示,桑叶多酚体外抑制蔗糖酶活性的半抑制浓度IC50为0.58 mg/mL,对DPPH自由基、羟自由基、过氧化氢的IC50分别为0.21、6.07、2.44 mg/mL。细胞试验显示,桑叶多酚能够增加高糖诱导的HepG2细胞对葡萄糖的消耗,促进葡萄糖的吸收,降低细胞内活性氧含量,提高了胰岛素信号通路中IR和IRS1的基因表达,在蛋白质水平提高了AKT蛋白的磷酸化,并抑制了糖异生蛋白PGC-1α的基因表达。另外,桑叶多酚还显著提高了脂代谢相关基因C/EBPα、PPARα和Visfatin的表达。以上结果提示,桑叶多酚能够抑制α-葡萄糖苷酶活性,清除自由基,激活肝细胞AKT信号通路,抑制葡萄糖异生,调控脂代谢,从而促进葡萄糖吸收代谢发挥降糖作用。研究结果将为桑叶多酚的功能研究提供基础数据,为桑叶活性物质的开发利用提供参考依据。Mulberry leaf has been used to treat diabetes since ancient times,and modern medical research has also proved that mulberry leaf has hypoglycemic effect.In this study,polyphenols were extracted from mulberry leaf.The hypoglycemic effect and mechanism of mulberry leaf polyphenols were studied by means of sucrase inhibition test,free radical scavenging test and insulin resistance test induced by high glucose in HepG2 hepatocytes.The results showed that the half inhibitory concentration(IC 50)of mulberry leaf polyphenols for inhibiting sucrase activity in vitro was 0.58 mg/mL.The IC 50 of mulberry leaf polyphenols on DPPH free radical,hydroxyl free radical and hydrogen peroxide were 0.21,6.07 and 2.44 mg/mL,respecti-vely.Experiments in cells showed that mulberry leaf polyphenols could increase glucose consumption,promote glucose absorption and reduce intracellular reactive oxygen species content in HepG2 cells induced by high glucose,and it increased the gene expression of IR and IRS1 in insulin signaling pathway,increased the phosphorylation of AKT at the protein level,and inhibited the gene expression of gluconeogenic protein PGC-1α.In addition,mulberry leaf polyphenols also significantly increased the expression of lipid metabolism gene C/EBPα,PPARαand Visfatin.These results suggest that mulberry leaf polyphenols can inhibitα-glucosidase activity,remove free radicals,activate the AKT signaling pathway of hepatocytes,inhibit gluconeogenesis,regulate lipid metabolism,promote glucose absorption and metabolism,and exert hypoglycemic effect.This study will provide basic data for the functional study of polyphenols in mulberry leaf,and provide reference for the development and utilization of active substances in mulberry leaf.

关 键 词：桑叶 多酚 降糖 胰岛素抵抗 HepG2肝细胞 

分 类 号：R285[医药卫生—中药学] S886.9[医药卫生—中医学]