Inhibition of viability of human retinal microvascular endothelial cells by vialinin A under high glucose condition  

作　　者：Zhi-Gang Chen Gao-Qin Liu Wei-Ming Liu Pei-Rong Lu 

机构地区：[1]Department of Ophthalmology,the First Affiliated Hospital of Soochow University,Suzhou 215006,Jiangsu Province,China [2]Jiangsu Clinical Immunology Institute,the First Affiliated Hospital of Soochow University,Suzhou 215006,Jiangsu Province,China

出　　处：《International Journal of Ophthalmology(English edition)》2024年第10期1809-1815,共7页国际眼科杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.81970830);Jiangsu Provincial Medical Innovation Team(No.CXTDA2017039).

摘　　要：AIM:To investigate the effects of vialinin A on viability of human retinal endothelial cells(HRECs)under high glucose condition and its potential mechanism.METHODS:The HRECs were divided into four groups:normal glucose control group(NG,5 mmol/L D-glucose),high glucose group(HG,30 mmol/L D-glucose),HG+1μmol/L vialinin A group,and HG+5μmol/L vialinin A group.The cell viabilities were measured with cell counting kit-8(CCK-8)assay for proliferation,with scratch assay for migration,and tube formation,for evaluation of the impact of vialinin A on cellular behaviour.Real-time PCR and Western blotting were used to determine the expression level of vascular endothelial growth factor(VEGF).RESULTS:The proliferative capacity and migration of HRECs was reduced by 5μmol/L vialinin A in high glucose environment(both P<0.05).Vialinin A also inhibited highglucose-induced tube formation of HRECs.The expression level of VEGF and PI3K in HRECs was also significantly decreased by vialinin A(P<0.05).CONCLUSION:Vialinin A inhibits the cell viability of HRECs.It may serve as a potential target for anti-angiogenic therapy.

关 键 词：vialinin A vascular endothelial growth factor human retinal endothelial cells cell viability 

分 类 号：R774.1[医药卫生—眼科]