罗汉果甜苷与黄芪多糖联用对小鼠骨骼肌抗氧化能力的影响  

10Effects of mogrosides and Astragalus polysacharin combination on antioxidant capacity of mouse skeletal muscle

作　　者：卢晓凤 骆隽言 韦明涵 农彩密 党钊林 李银 王倩 蒋钦杨[1] 黄艳娜[1] LU Xiao-feng;LUO Jun-yan;WEI Ming-han;NONG Cai-mi;DANG Zhao-lin;LI Yin;WANG Qian;JIANG Qin-yang;HUANG Yan-na(College of Animal Science and Technology,Guangxi University,Nanning,Guangxi 530004,China)

机构地区：[1]广西大学动物科学技术学院,广西南宁530004

出　　处：《南方农业学报》2024年第7期1925-1934,共10页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(32360839,31760672);国家级大学生创新创业训练计划项目(202310593044)。

摘　　要：【目的】探讨罗汉果甜苷与黄芪多糖联用对提升小鼠骨骼肌抗氧化能力的影响,并揭示其协同作用机制,为开发罗汉果甜苷和黄芪多糖作为绿色、安全的新型饲料添加剂应用于畜禽骨骼肌抗氧化能力提升及肉品质改善提供理论依据。【方法】将40只SPF级昆明小鼠随机分为4组,即对照组(CK)、罗汉果甜苷组(MOG)、黄芪多糖组(APS)及罗汉果甜苷+黄芪多糖联用组(MOG+APS),每组10只。采取经口灌胃方式给药,MOG组小鼠每日灌服200 mg/kg罗汉果甜苷,APS组小鼠每日灌服200 mg/kg黄芪多糖,MOG+APS组小鼠每日灌服200 mg/kg罗汉果甜苷+200 mg/kg黄芪多糖,CK组小鼠每日灌服0.1 mL生理盐水,连续灌服4周。给药结束后,采集小鼠脏器计算脏器指数,测定血清和骨骼肌中的抗氧化酶活性,并通过实时荧光定量PCR检测小鼠骨骼肌中抗氧化相关基因及Keap1/Nrf2信号通路相关基因的表达变化。【结果】与CK组小鼠相比,MOG组、APS组和MOG+APS组小鼠的平均日采食量、平均日增重及各脏器指数均无显著变化(P>0.05)。MOG组、APS组和MOG+APS组小鼠血清和腓肠肌中的丙二醛(MDA)含量较CK组小鼠显著降低(P<0.05,下同),谷胱苷肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性则显著升高;MOG+APS组小鼠血清和腓肠肌中的MDA含量较MOG组和APS组显著降低,而GSH-Px和SOD活性显著升高。与MOG组和APS组小鼠相比,MOG+APS组小鼠腓肠肌中的GSH-Px和SOD基因相对表达量显著上升,NF-E2相关因子2基因(Nrf2)相对表达量分别显著升高19.02%和20.10%,而Kelch样环氧氯丙烷相关蛋白1基因(Keap1)相对表达量分别显著下降29.92%和24.20%。【结论】罗汉果甜苷与黄芪多糖联用能激活Keap1/Nrf2信号通路,上调SOD和GSH-Px基因表达,提高抗氧化酶活性,而增强小鼠骨骼肌抗氧化能力。罗汉果甜苷和黄芪多糖可作为绿色、安全的新型饲料添加剂,用于提升畜禽骨骼肌抗氧化能力及�【Objective】To explore the effect of mogrosides combined with Astragalus polysacharin on improving the antioxidant capacity of skeletal muscle in mice,and to reveal its synergistic mechanism,so as to provide theoretical basis for the development of mogrosides and Astragalus polysacharin as green and safe new feed additives for improving the antioxidant capacity of skeletal muscle and meat quality in livestock and poultry.【Method】Forty SPF KM mice were randomly divided into four groups:the control(CK)group,the mogrosides(MOG)group,the Astragalus polysacharin(APS)group,and the mogrosides+Astragalus polysacharin combination(MOG+APS)group,with 10 mice in each group.The drug was administered by oral gavage,the mice in the MOG group were given 200 mg/kg mogrosides daily,the mice in the APS group were given 200 mg/kg Astragalus polysacharin daily,the mice in the MOG+APS group were given 200 mg/kg mogrosides+200 mg/kg Astragalus polysacharin daily,and the mice in the CK group were given 0.1 ml of normal saline daily for 4 weeks.After the administration,the organs of mice were collected to calculate the organ index,the antioxidant enzyme activities in serum and skeletal muscle were determined,and the expression changes of antioxidant-related genes and Keap1/Nrf2 signaling pathway-related genes in skeletal muscle of mice were detected by real-time fluorescence quantitative PCR.【Result】Compared with CK group,the average daily feed intake,average daily gain and organ index of MOG group,APS group and MOG+APS group had no significant changes(P>0.05).The content of malondialdehyde(MDA)in serum and gastrocnemius muscle of mice in MOG group,APS group and MOG+APS group was significantly decreased compared to that in CK group(P<0.05,the same below),while the activities of glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)were significantly increased.The content of MDA in serum and gastrocnemius muscle of mice in MOG+APS group was significantly decreased compared to that in MOG group and APS group,while the activ

关 键 词：小鼠 罗汉果甜苷 黄芪多糖 骨骼肌 抗氧化能力 Keap1/Nrf2信号通路 

分 类 号：S816.7[农业科学—饲料科学]