Injectable Nanorobot-Hydrogel Superstructure for Hemostasis and Anticancer Therapy of Spinal Metastasis  

作　　者：Qing Chen Miao Yan Annan Hu Bing Liang Hongwei Lu Lei Zhou Yiqun Ma Chao Jia Dihan Su Biao Kong Wei Hong Libo Jiang Jian Dong 

机构地区：[1]Department of Orthopaedic Surgery,Zhongshan Hospital,Fudan University,Shanghai 200032,People’s Republic of China [2]Department of Chemistry,Fudan University,Shanghai 200438,People’s Republic of China [3]Department of Surgery,Yong Loo Lin School of Medicine,National University of Singapore,1E Kent Ridge Road,Singapore 119228,Singapore [4]Department of Geriatrics and Gerontology,Huadong Hospital,Fudan University,Shanghai 200438,People’s Republic of China [5]State Key Laboratory of Molecular Engineering of Polymers,Fudan University,Shanghai 200438,People’s Republic of China [6]Department of Orthopaedic Surgery Zhongshan Hospital Wusong Branch,Fudan University,Shanghai 200940,People’s Republic of China

出　　处：《Nano-Micro Letters》2024年第12期59-80,共22页纳微快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.52103171,82172738,82272457,22305044);China Postdoctoral Science Foundation(2023M730638);“Technology Innovation Action Plan”of Science and Technology Commission of Shanghai Municipality(21S11902700);Natural Science Foundation of Shanghai(21ZR1412300),Shanghai Science and Technology program(23Y31900202,23010502600);Shanghai“Rising Stars of Medical Talent”Youth Development Program(Youth Medical Talents-Specialist Program,[2020]087);Medical Engineering fund of Fudan University(yg2023-27).

摘　　要：Surgery remains the standard treatment for spinal metastasis.However,uncontrolled intraoperative bleeding poses a significant challenge for adequate surgical resection and compromises surgical outcomes.In this study,we develop a thrombin(Thr)-loaded nanorobothydrogel hybrid superstructure by incorporating nanorobots into regenerated silk fibroin nanofibril hydrogels.This superstructure with superior thixotropic properties is injected percutaneously and dispersed into the spinal metastasis of hepatocellular carcinoma(HCC)with easy bleeding characteristics,before spinal surgery in a mouse model.Under near-infrared irradiation,the self-motile nanorobots penetrate into the deep spinal tumor,releasing Thr in a controlled manner.Thr-induced thrombosis effectively blocks the tumor vasculature and reduces bleeding,inhibiting tumor growth and postoperative recurrence with Au nanorod-mediated photothermal therapy.Our minimally invasive treatment platform provides a novel preoperative therapeutic strategy for HCC spinal metastasis effectively controlling intraoperative bleeding and tumor growth,with potentially reduced surgical complications and enhanced operative outcomes.

关 键 词：Silk fibroin Nanofibril hydrogels Nanorobots THROMBIN Spinal metastasis 

分 类 号：TP242[自动化与计算机技术—检测技术与自动化装置] TB383.1[自动化与计算机技术—控制科学与工程] R318[一般工业技术—材料科学与工程]