circMBOAT2靶向miR-526b-3p对肺癌A549细胞增殖和凋亡的影响  

Effect of circMBOAT2 targeting miR-526b-3p on the proliferation and apoptosis of lung cancer A549 cells

作　　者：谌登珍 马强 Chen Dengzhen;Ma Qiang(Department of Respiratory Medicine,Shangluo Central Hospital,Shangluo 726000,China;Department of Respiratory and Critical Care Medicine,Shangluo Central Hospital,Shangluo 726000,China)

机构地区：[1]商洛市中心医院呼吸内科,商洛726000 [2]商洛市中心医院呼吸与危重症医学科,商洛726000

出　　处：《国际医药卫生导报》2024年第20期3363-3368,共6页International Medicine and Health Guidance News

基　　金：陕西省重点研发计划(2020SF-116)。

摘　　要：目的探讨circMBOAT2是否通过靶向miR-526b-3p影响肺癌A549细胞的增殖和凋亡。方法收集2021年6月至2023年9月在商洛市中心医院行手术治疗的47份新鲜肺癌组织和癌旁组织。实时定量PCR(qRT-PCR)测定肺癌组织中circMBOAT2和miR-526b-3p表达。肺癌A549细胞分为Con组、si-NC组、si-circMBOT2组、pcDNA组、pcDNA-circMBOAT2组、miR-NC组、miR-526b-3p组、si-circMBOAT2+anti-miR-NC组、si-circMBOAT2+anti-miR-526b-3p组。运用CCK-8、流式细胞术和免疫印迹实验分别测定细胞增殖、凋亡与Ki-67、Bcl-2、Bax蛋白表达。双荧光素酶报告基因检测分析circMBOAT2与miR-526b-3p靶向。统计学方法采用t检验、SNK-q检验。结果肺癌组织中circMBOAT2表达高于癌旁组织[(4.25±0.22)比(1.00±0.05)],miR-526b-3p表达低于癌旁组织[(0.33±0.04)比(1.00±0.07)],差异均有统计学意义(t=98.76、56.97,均P<0.05)。干扰circMBOT2或过表达miR-526b-3p能抑制肺癌A549细胞增殖及Ki-67、Bcl-2蛋白表达,并增加凋亡率、Bax蛋白表达(均P<0.05)。circMBOAT2靶向调控miR-526b-3p的表达,抑制miR-526b-3p表达逆转了干扰circMBOAT2表达对肺癌A549细胞增殖和凋亡的作用。结论circMBOAT2在肺癌组织中高表达,通过靶向调控miR-526b-3p表达,促进肺癌A549细胞的增殖和抑制凋亡。Objective To investigate whether circMBOAT2 affects the proliferation and apoptosis of lung cancer A549 cells by targeting miR-526b-3p.Methods A total of 47 fresh lung cancer tissues and para-cancer tissues were collected from Shangluo Central Hospital from June 2021 to September 2023.Real-time quantitative PCR(qRT-PCR)was used to determine the expressions of circMBOAT2 and miR-526b-3p in lung cancer tissues.Lung cancer A549 cells were divided into a Con group,a si-NC group(transfected with si-NC),a si-circMBOT2 group(transfected with si-circMBOT2),a pcDNA group(transfected with pcDNA),a pcDNA-circMBOAT2 group(transfected with pcDNA-circMBOAT2),a miR-NC group(transfected with miR-NC),a miR-526b-3p group(transfected with miR-526b-3p mimics),a si-circMBOAT2+anti-miR-NC group(co-transfected with si-circMBOAT2 and anti-miR-NC),a si-circMBOAT2+anti-miR-526b-3p group(co-transfected with si-circMBOAT2 and miR-526b-3p inhibitor).Cell proliferation,apoptosis,and protein expressions of Ki-67,Bcl-2,and Bax were measured by CCK-8,flow cytometry,and Western blotting.Dual luciferase reporter gene detection was used to analyze the targeting relationship between circMBOAT2 and miR-526b-3p.t test and SNK-q test were used.Results The expression of circMBOAT2 in the lung cancer tissues was higher than that in the para-cancer tissues[(4.25±0.22)vs.(1.00±0.05)],and the expression of miR-526b-3p was lower than that in the para-cancer tissues[(0.33±0.04)vs.(1.00±0.07)],with statistically significant differences(t=98.76 and 56.97,both P<0.05).Interference with circMBOT2 or overexpression of miR-526b-3p inhibited the proliferation and protein expressions of Ki-67 and Bcl-2 of lung cancer A549 cells,and increased apoptosis rate and Bax protein expression(all P<0.05).circMBOAT2 targeted to regulate the expression of miR-526b-3p,and inhibition of miR-526b-3p expression reversed the effect of interfering circMBOAT2 expression on the proliferation and apoptosis of lung cancer A549 cells.Conclusion circMBOAT2 is highly expressed in lung ca

关 键 词：肺癌 circMBOAT2 miR-526b-3p 增殖 凋亡 

分 类 号：R734.2[医药卫生—肿瘤]