冷却贮藏期间滩羊肉氧化应激与线粒体功能损伤的关系  

Relationship between Oxidative Stress and Impairment of Mitochondrial Function in Tan Sheep Meat during Cooling Storage

作　　者：魏志宝 罗瑞明[1] 高爽 杨东松 虎瑶 张赫宇 马佳荣 WEI Zhibao;LUO Ruiming;GAO Shuang;YANG Dongsong;HU Yao;ZHANG Heyu;MA Jiarong(School of Food Science and Engineering,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学食品科学与工程学院,宁夏银川750021

出　　处：《食品科学》2024年第19期201-207,共7页Food Science

基　　金：国家自然科学基金地区科学基金项目(32260555)。

摘　　要：研究宰后冷却贮藏时间中氧化应激和线粒体损伤之间的相互关系,以冷却贮藏期间滩羊背最长肌(M.longissimus dorsi)为研究对象,设置N-乙酰-L-半胱氨酸(N-acetyl-L-cysteine,NAC)抑制组和生理盐水对照组,分别测定两个组冷却贮藏0、2、4、6、8 d时的线粒体膜通透性转换孔(mitochondrial permeability transition pore,MPTP)开放程度、膜电位、活性氧(reactive oxygen species,ROS)含量、复合物(Ⅰ、Ⅱ、Ⅲ、Ⅳ)活性、琥珀酸脱氢酶(succinate dehydrogenase,SDH)活性、过氧化氢酶(catalase,CAT)活性、超氧化物歧化酶(superoxide dismutase,SOD)活性、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性以及丙二醛(malondialdehyde,MDA)含量的变化情况,并对其肌细胞线粒体微观结构进行观察,分析其变化情况。结果表明:在成熟过程中,抑制组和对照组的滩羊肉肌细胞线粒体的MPTP开放程度逐渐增大(P<0.05);ROS、MDA含量呈现上升趋势(P<0.05);线粒体膜电位和复合物(Ⅰ、Ⅱ、Ⅲ、Ⅳ)、SDH、CAT、SOD、GSH-Px活性呈现逐渐下降的趋势(P<0.05)。结论:滩羊在宰杀后,其肌肉组织中发生氧化应激反应,导致ROS含量显著增高,超出机体自身抗氧化防御清除过量ROS能力时,进而导致氧化还原系统失衡,而氧化还原系统的失衡进一步加速肌体内ROS水平的上升,从而引发MPTP开放和线粒体膜电位降低,严重损害线粒体,然而NAC可以缓解此类现象;ROS水平的上升会破坏线粒体电子传递链的正常运行,导致线粒体损伤加重;同时脂质氧化产物MDA也能损伤线粒体功能结构,导致线粒体发生肿胀、变形等,从而引起其生物功能紊乱。To study the relationship between oxidative stress and mitochondrial damage during post-slaughter chilling,M.longissimus dorsi from Tan sheep was treated with the antioxidant N-acetyl-L-cysteine(NAC)solution or physiological saline and stored at 0–4℃.After 0,2,4,6 and 8 days,the opening degree of mitochondrial permeability transition pore(MPTP),membrane potential,reactive oxygen species(ROS)content,complexⅠ,Ⅱ,ⅢandⅣactivities,the activities of succinate dehydrogenase(SDH),catalase(CAT),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)content were determined,and the mitochondrial structure of myoblasts was observed under transmission electron microscopy(TEM).The results showed that the opening degree of MPTP in both the NAC and control groups gradually increased(P<0.05)during the postmortem maturation process,and so did the ROS and MDA levels(P<0.05).The mitochondrial membrane potential and the activities of complexesⅠ,Ⅱ,ⅢandⅣ,SDH,CAT,SOD and GSH-Px showed a gradual decreasing trend(P<0.05).It can be concluded that oxidative stress occurred in the muscle tissue of Tan sheep after slaughter,resulting in a significant increase in ROS content.Excessive ROS,exceeding the ability of the antioxidant defense system to scavenge it,could lead to an imbalance in the redox system,which could in turn accelerate the increase of ROS levels,thereby triggering the opening of MPTP and a decrease in the mitochondrial membrane potential,finally resulting in serious mitochondrial damage.However,NAC alleviated this phenomenon.Increased ROS levels impaired the normal operation of the mitochondrial electron transport chain,thus leading to increased mitochondrial damage.In addition,the lipid oxidation product MDA also damaged the function and structure of mitochondria,causing mitochondrial structural damages such as swelling and deformation,and finally leading to biological dysfunctions.

关 键 词：滩羊 氧化应激 线粒体 功能损伤 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]