机械敏感离子通道Piezo1在压力侧正畸牙槽骨改建的动物实验研究  

Role of mechanosensitive ion channel Piezo1 in compression-side orthodontic alveolar bone remodeling in rats

作　　者：杜育桂 何正权[1] 张一丹 白琳娜 李健怡 杨凯[1] DU Yu-gui;HE Zheng-quan;ZHANG Yi-dan;BAI Lin-na;LI Jian-yi;YANG Kai(Department of Orthodontics,Capital Medical University School of Stomatology,Beijing 100050,China)

机构地区：[1]首都医科大学口腔医学院正畸科,北京100050

出　　处：《北京口腔医学》2024年第4期229-234,共6页Beijing Journal of Stomatology

基　　金：北京市自然科学基金(7222077);北京市自然科学基金-海淀原始创新联合基金(L232106)。

摘　　要：目的探讨机械敏感性离子通道Piezo1在大鼠正畸牙移动过程中压力侧牙槽骨中的表达及作用,并探究可能的信号通路。方法建立正畸牙移动模型,对不同时间点压力侧牙周组织中机械敏感离子通道Piezo1、破骨细胞数目、破骨细胞标记物(RANKL、OPG)、成骨标记物(Runx2、Osterix)和Wnt非经典信号通路(Wnt5a、CAMKⅡ)的表达水平进行检测并分析其变化规律。结果在正畸牙移动过程中,压力侧破骨细胞数目增加,RANKL表达增加;Piezo1、OPG、Runx2、Osterix、Wnt5a及CAMKⅡ的表达先增高后下降。结论Piezo1可能参与了正畸牙移动过程中压力侧的牙槽骨改建,并通过Wnt非经典信号通路发挥作用。Objective To investigate the expression and role of the mechanosensitive ion channel Piezo1 in the compression-side alveolar bone during orthodontic tooth movement(OTM)in rats,and to explore the possible signaling pathways.Methods The OTM model was established,and the expression levels of the mechanosensitive ion channel Piezo1,osteoclasts number,osteoblast markers(RANKL,OPG),osteogenic markers(Runx2,Osterix)and the Wnt non-classical signaling pathway(Wnt5a,CAMKII)were examined in the compression-side periodontal tissues at different time points.Results During OTM process,the osteoclasts number and RANKL expression increased;Piezo1,OPG,Runx2,Osterix,Wnt5a and CAMKⅡlevels decreased significantly after the initial increase.Conclusions Piezo1 may be involved in the remodeling of the alveolar bone on the compression side during OTM process and may function through the Wnt non-classical signaling pathway.

关 键 词：机械敏感性离子通道Piezo1 正畸牙移动 骨改建 

分 类 号：R783.5[医药卫生—口腔医学]