体外法研究异绿原酸对绵羊日粮养分消化与瘤胃发酵的影响  

In vitro study on effects of isochlorogenic acid supplementation in sheep diets on nutrient digestion and rumen fermentation

作　　者：李淑艳 焦婷[1] 齐帅 张霞 王虎宁 赵生国[2] LI Shuyan;JIAO Ting;QI Shuai;ZHANG Xia;WANG Huning;ZHAO Shengguo(Key Laboratory for Grassland Ecosystem,Ministry of Education,Sino-US Grassland Animal Husbandry Sustainable Development Research Center,Pratacultural College,Gansu Agricultural University,Lanzhou 730070,China;College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,中美草地畜牧业可持续发展研究中心,甘肃兰州730070 [2]甘肃农业大学动物科学技术学院,甘肃兰州730070

出　　处：《浙江农业学报》2024年第8期1753-1763,共11页Acta Agriculturae Zhejiangensis

基　　金：甘肃省高校创新基金项目(2022B-096);甘肃省科技计划(技术创新引导计划-鲁甘科技协作专题)项目(22CX2NA005)。

摘　　要：在绵羊基础日粮中添加0.03%、0.06%、0.09%、0.12%、0.20%的异绿原酸(依次标记为T1~T5处理),并以不添加异绿原酸作为对照(CK),开展体外发酵试验。在体外培养2、6、12、24、48 h后,研究不同处理对绵羊日粮养分降解和瘤胃发酵特性的影响。结果表明:发酵48 h时,T3、T4、T5处理的产气量显著(P<0.05)高于其他处理。发酵2、48 h时,T4处理的日粮体外干物质降解率显著高于CK。发酵2、24、48 h时,T4和T5处理的发酵液pH值显著低于CK。发酵12 h时,T3、T4、T5处理的丁酸和总挥发性脂肪酸浓度均显著高于CK。发酵48 h时,T3、T4处理的乙酸-丙酸浓度比显著高于CK,但其氨态氮质量浓度显著低于CK。综上,添加适量的异绿原酸可显著提高日粮的体外干物质降解率,及发酵液中的丁酸和总挥发性脂肪酸浓度,根据饲料的综合组合效应指数值确定的建议添加量为0.09%~0.12%。In the present in vitro fermentation experiment,the treatments T1-T5 with 0.03%,0.06%,0.09%,0.12%and 0.20%isochlorogenic acid(ICGA)added to the sheep basic diet were set,as well as a blank control without addition of ICGA.The effects of ICGA addition on diet nutrient degradation and sheep rumen fermentation characteristics were explored at 2,6,12,24,48 hours in vitro culture.The results showed that the gas production under T3,T4 and T5 treatments was significantly(P<0.05)higher than that under the other treatments at 48 h of fermentation.At 2,48 h of fermentation,the in vitro dry matter degradation rate under T4 treatment was significantly higher than that under CK.At 2,24,48 h of fermentation,the pH value of the fermentation fluid under T4 and T5 treatments was significantly lower than that under CK.After 12 h of fermentation,the concentrations of butyric acid and total volatile fatty acids under T3,T4 and T5 treatments were significantly higher than those under the CK.At 48 h of fermentation,the ratio of acetate concentration to propionate concentration under T3 and T4 treatments was significantly higher than that under the CK,while the ammoniacal nitrogen mass concentration under T3 and T4 treatments was significantly lower than that under the CK.In conclusion,addition of appropriate amount of ICGA could significantly increase the in vitro dry matter degradation rate of sheep diet,the concentratin of butyrate and total volatile fatty acids in the fermentation fluid.According to the multiple factors associative effects index,the recommended ICGA addition amount was 0.09%-0.12%.

关 键 词：酚酸类化合物 异绿原酸 瘤胃 体外发酵 养分消化 饲料组合效应 

分 类 号：S816.7[农业科学—饲料科学]