副干酪乳酪杆菌Glory LP16对氨苄青霉素导致BALB/c小鼠肠道菌群紊乱的恢复作用  

Restorative effect of ampicillin-induced intestinal flora disorders by Lactobacillus paracasei Glory LP16 in BALB/c mice

作　　者：马微微[1] 吴金富 赵伊阳 张紫薇 黄莉莉[1] MA Weiwei;WU Jinfu;ZHAO Yiyang;ZHANG Ziwei;HUANG Lili(School of Pharmacy,Heilongjiang University of Traditional Chinese Medicine,Harbin 150000,China)

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150000

出　　处：《中国乳品工业》2024年第9期22-27,49,共7页China Dairy Industry

基　　金：黑龙江省博士后科研启动金(LBH-Q13161);黑龙江省自然科学基金(LH2019H106);黑龙江中医药中青年科技攻关项目(ZQG-042)。

摘　　要：利用动物模型评价不同浓度副干酪乳酪杆菌对肠道菌群失调的改善作用,并且研究剂量效果。将60只BALB/c小鼠分成5组,通过灌胃氨苄青霉素构建肠道菌群紊乱的模型,同时将副干酪乳酪杆菌Glory LP16以浓度为区分依据,分为对照组、模型组、低剂量组(1.6×10^(6)CFU/只)、中剂量组(1.6×10^(7)CFU/只)和高剂量组(1.6×10^(8)CFU/只),测量小鼠体质量变化,收集小鼠的结肠组织和粪便样本进行长度测量、组织病理学分析、肠道屏障、肠道通透性、肠道微生物群分析以及短链脂肪酸测定。结果表明,与模型组相比副干酪乳酪杆菌Glory LP16喂养组显著增加双歧杆菌、乳杆菌等有益菌的相对丰度,恢复结肠组织结构,增加肠道屏障相关基因的表达,提高短链脂肪酸含量且在中剂量条件下能够很好地维持肠道通道通透性,保持肠道完整性,恢复氨苄青霉素导致的BALB/c小鼠肠道菌群紊乱。The aim of this study was to evaluate the ameliorative effect of different concentrations of Lactobacillus paracasei on intestinal dysbiosis using an animal model and to investigate the dose effect.Sixty BALB/c mice were divided into five groups,and the model of intestinal flora disorders was constructed by gavage of ampicillin,while the concentration of Lactobacillus paracasei Glory LP16 was used as the basis of differentiation,and the mice were divided into the control group,the model group,the low-dose group(1.6×10^(6)CFU/only),the middle-dose group(1.6×10^(7)CFU/only),and the high-dose group(1.6×10^(8)CFU/only),body weight changes were measured,and colon tissue and faecal samples were collected for length measurements,histopathological analyses,intestinal barrier,intestinal permeability,intestinal microbiota analysis,and short-chain fatty acid determinations.Compared with the model group,the Lactobacillus paracasei Glory LP16-fed group significantly increased the relative abundance of beneficial bacteria such as Bifidobacterium and Lactobacillus,restored the colonic tissue structure,increased the expression of intestinal barrier-related genes,increased the content of short-chain fatty acids and maintained the intestinal channel permeability well enough to preserve the intestinal integrity under the medium-dose condition,restored the ampicillin-induced BALB/c mice with disturbed intestinal flora.

关 键 词：副干酪乳酪杆菌Glory LP16 肠道菌群 肠道屏障 短链脂肪酸 

分 类 号：TS201.3[轻工技术与工程—食品科学]