HNRNPA1基因在结直肠癌组织中高表达及其潜在的诊断和治疗价值  

HNRNPA1 gene is highly expressed in colorectal cancer:its prognostic implications and potential as a therapeutic target

作　　者：纪凯 于冠宇 周乐其 张天帅 凌潜龙 满文江 朱冰 张卫 JI Kai;YU Guanyu;ZHOU Leqi;ZHANG Tianshuai;LING Qianlong;MAN Wenjiang;ZHU Bing;ZHANG Wei(Department of Gastrointestinal Surgery,First Affiliated Hospital of Bengbu Medical University,Bengbu 233000,China;Department of Colorectal Surgery,Shanghai Changhai Hospital,Naval Medical University,Shanghai 200082,China)

机构地区：[1]蚌埠医科大学第一附属医院胃肠外科,安徽蚌埠233000 [2]海军军医大学第一附属医院//上海长海医院肛肠外科,上海200082

出　　处：《南方医科大学学报》2024年第9期1685-1695,共11页Journal of Southern Medical University

基　　金：国家自然科学基金(82072750);安徽省高校科学研究重大项目(KJ2021ZD0090);促进市级医院临床技能与临床创新三年行动计划-研究型医师创新转化能力培训项目(SHDC2022CRT007);蚌埠医学院研究生科研创新项目(Byycx22090);安徽省2023年度新时代育人省级质量工程项目(2023xscx125)。

摘　　要：目的通过生物信息学和细胞实验探究HNRNP A1在结直肠癌中的临床意义及其在肿瘤组织中的表达情况。方法使用HPA、TIMER和GEPIA数据库,分析HNRNP A1在结直肠癌组织中的表达水平,并检验HNRNP A1与Ki-67/VEGFA在结直肠癌中表达的相关性。使用Kaplan-Meier Plotter数据库评估HNRNP A1 mRNA水平与结直肠癌患者生存率之间的联系。通过基因富集途径分析,预测HNRNP A1在结直肠癌中的潜在生物学作用。通过免疫组织化学(IHC)和Western blotting技术检测HNRNP A1在结直肠癌及其癌旁组织中的蛋白表达。利用TIMER数据库网站对HNRNP A1在免疫浸润细胞中的表达进行预测。使用慢病毒敲低RKO/Caco2细胞中HNRNP A1的表达;通过CCK-8实验检测细胞增殖,使用克隆形成实验检测HNRNP A1对细胞增殖能力的影响;利用细胞划痕实验和Transwell迁移实验评估两组细胞(RKO/Caco2-nc、RKO/Caco2-sh)的迁移能力。最后验证HNRNP A1小分子抑制剂(VPC-80051)对肿瘤细胞增殖的影响。结果在结直肠癌(CRC)肿瘤组织中HNRNP A1的表达显著上调并与患者的不良预后显著相关(P<0.01)。TIMER数据库的分析结果指出,HNRNP A1与肿瘤微环境中的免疫细胞之间存在一定的相关性。根据GEPIA数据库的分析,CRC组织中HNRNP A1、MKI67和VEGFA的表达均较高(P<0.05),且HNRNP A1与这两者之间存在正相关关系。通过Kaplan-Meier Plotter进行的生存分析表明,在CRC中,HNRNP A1的高表达预示着较差的总生存期(P=0.0081)和无进展生存期(P=0.012)。基因富集通路分析的数据显示,HNRNP A1可能参与到多个与CRC进展相关的生物途径中。HNRNP A1影响RKO/Caco2细胞的增殖和迁移能力,对照组(RKO/Caco2-nc)的增殖能力、克隆形成能力和迁移能力均优于实验组(RKO/Caco2-sh),差异有统计学意义(P<0.05);HNRNP A1小分子抑制剂(VPC-80051)可以有效抑制结直肠癌增殖活性,并具有时间和浓度依赖性;IHC显示HNRNP A1在结直肠癌中高Objective To investigate the expression level of HNRNP A1 in colorectal cancer(CRC)and its prognostic implications.Methods We investigated HNRNP A1 expression level in CRC using HPA,TIMER,and GEPIA databases and analyzed its association with Ki-67 and VEGFA expressions.Kaplan-Meier Plotter database was used to analyze the correlation of HNRNP A1 mRNA levels with the survival rates of CRC patients.Pathway enrichment analysis was performed for predicting the biological roles of HNRNP A1 in CRC progression.Immunohistochemistry and Western blotting were used to examine the protein levels of HNRNP A1 in CRC versus adjacent tissues,and TIMER was used for assessing its expression in the infiltrating immune cells.In RKO/Caco2 cells,the effects of lentivirus-mediated knockdown of HNRNP A1 on cell proliferation and migration were observed,and the inhibitory effect of VPC-80051(a HNRNP A1 inhibitor)on cell proliferation was evaluated to assess its potential as a therapeutic agent.Results HNRNP A1 was significantly overexpressed in CRC tissues and correlated with a poor prognosis of the patients.HNRNP A1 expression level was correlated with the infiltrating immune cells in CRC microenvironment and positively correlated with MKI67 and VEGFA expressions in CRC.A high HNRNP A1 expression predicted a in survival and progression-free survival of CRC patients and was involved in multiple biological processes related with CRC progression.In RKO/Caco2 cells,HNRNP A1 knockdown significantly suppressed cell proliferation and migration,and treatment with VPC-80051 also effectively inhibited CRC cell proliferation.Immunohistochemical study demonstrated a close correlation of HNRNP A1 overexpression with tumor stage of CRC.Conclusion HNRNP A1 is overexpressed in CRC tissues to modulate cell proliferation and migration and is correlated with a poorer prognosis.VPC-80051 can effectively inhibit CRC cell proliferation,suggesting the potential of HNRNP A1 as a therapeutic target for CRC.

关 键 词：结直肠癌 HNRNP A1 增殖与转移 预后 生物信息学 

分 类 号：R735.34[医药卫生—肿瘤]