超高效液相色谱-四极杆串联离子阱质谱法同时检测动物源性食品中26种新型抗病毒药物  

Simultaneous determination of 26 kinds of antiviral drugs in animal derived foods by ultra performance liquid chromatography quadrupole tandem ion trap-mass spectrometry

作　　者：刘瑜 董鸿竺 王悦 耿庆华 李晓东 张彤 姜莉 万建春 李志远 LIU Yu;DONG Hong-Zhu;WANG Yue;GENG Qing-Hua;LI Xiao-Dong;ZHANG Tong;JIANG Li;WAN Jian-Chun;LI Zhi-Yuan(Technical Center of Shenyang Customs,Shenyang 110000,China;Technical Center of Jiangxi Customs,Jiangxi 330000,China;Shanghai AB Sciex Analytical Instrument Trading Co.,Ltd.,Beijing 100015,China)

机构地区：[1]沈阳海关技术中心,沈阳110000 [2]江西海关技术中心,江西330000 [3]上海爱博才思科技有限公司,北京100015

出　　处：《食品安全质量检测学报》2024年第17期101-107,共7页Journal of Food Safety and Quality

基　　金：海关总署科研项目(2022HK021);辽宁省自然科学基金项目(2022MS426)。

摘　　要：目的建立一种超高效液相色谱-四极杆串联离子阱质谱法(ultra performance liquid chromatography quadrupole tandem ion trap-mass spectrometry,UPLC-MS/MS)同时检测动物源性食品中多种抗病毒药物的方法。方法样品经三氯乙酸-乙腈(20:80,V:V)溶液提取,Captive EMR-Lipid净化柱净化,采用Waters BEH C18色谱柱分离,UPLC-MS/MS检测,多反应监测-信息关联扫描-增强子离子扫描(multiple response monitoring-information dependent acquisition-enhanced product ion,MRM-IDA-EPI)确证,外标法定量。结果26种抗病毒药物定量限为0.1~5.0μg/kg,相关系数均高于0.99,回收率范围为71%~97%,相对标准偏差为1.3%~10.7%。结论采用新型通过型净化技术及四极杆串联离子阱质谱法,可同时对26种抗病毒药物进行定性、定量检测;采用二级全扫描谱图与谱库检索比对,实现了化合物的确证分析。Objective To establish a method for the simultaneous determination of multiple antiviral drugs in animal derived foods by ultra performance liquid chromatography quadrupole tandem ion trap-mass spectrometry(UPLC-MS/MS).Methods The sample was extracted with a solution of trichloroacetic acid acetonitrile(20:80,V:V),purified with Captive EMR Lipid,separated using a Waters BEH C18 chromatographic column,detected by UPLC-MS/MS,confirmed by multiple response monitoring-information dependent acquisition-enhanced product ion(MRM-IDA-EPI),and quantified by external standard method.Results The limits of quantitative of 26 kinds of antiviral drugs were 0.1–5.0μg/kg,with correlation coefficients higher than 0.99.The recovery rates were 71%–97%,and the relative standard deviations were 1.3%–10.7%.Conclusion Using a new type of purification technology and quadrupole tandem ion trap mass spectrometry,26 kinds of antiviral drugs can be qualitatively and quantitatively detected simultaneously;by using a second level full scan spectrogram and comparing it with the spectral library retrieval,the confirmation analysis of compounds can be achieved.

关 键 词：抗病毒药物 超高效液相色谱-四极杆串联离子阱质谱法 动物源性食品 Captive EMR-Lipid 

分 类 号：O657.63[理学—分析化学] TS207.3[理学—化学]