木质素降解菌的筛选及其对酒糟的预处理研究  

Screening of Lignin-degrading Fungal Strain and Its Pretreatment of Distillers'Grains

作　　者：杨莉[1] 杨义 郑碧君 陈佳[1] 田永强[1] YANG Li;YANG Yi;ZHENG Bijun;CHEN Jia;TIAN Yongqiang(College of Biomass Science and Engineering,Sichuan University,Chengdu 610065,China)

机构地区：[1]四川大学轻工科学与工程学院,四川成都610065

出　　处：《生物质化学工程》2024年第5期45-52,共8页Biomass Chemical Engineering

基　　金：酶资源四川省科技共享服务平台(无编号)。

摘　　要：为解决生物质炼制过程中木质素的生物抗性,本研究筛选出一株木质素降解菌,用其对酒糟进行预处理,并使用傅里叶红外光谱仪、X射线衍射仪、扫描电子显微镜、Zeta电位与纳米粒度仪、低场核磁共振分析仪和热重分析仪对预处理前后酒糟的微观结构与理化性质进行表征。结果表明:这株木质素降解菌是栓菌属,将其命名为Trametes sp.SCU31(T.sp.SCU31)。T.sp.SCU31能够高效降解酒糟中的木质素,可使酒糟中33.86%酸不溶木质素与50.88%酸溶木质素发生降解。经T.sp.SCU31预处理后的酒糟表面布满孔洞与碎片,更多的纤维束暴露出来,显著增加了纤维素酶的可及性,这些结果表明T.sp.SCU31预处理能够高效降解木质素,破坏木质纤维的抗性。In order to address the biological resistance of lignin during biomass refining,this study selected a lignin-degrading fungal that was used for the distillers'grains pretreatment.Fourier infrared spectrometer,X-ray diffractometer,scanning electron microscopy,Zeta potential and nanoparticle size analyzer,low-field nuclear magnetic resonance analyzer,and thermogravimetric analyzer were used to characterize the microstructure and physicochemical properties of distillers'grains before and after preconditioning.The results showed that this lignin-degrading strain was the genus Trametes,named Trametes sp.SCU31(T.sp.SCU31).T.sp.SCU31 was able to efficiently degrade lignin in distillers'grains,which could degrade acid insoluble lignin and acid soluble lignin by 33.86%and 50.88%,respectively.The surface of distillers'grains pretreated by T.sp.SCU31 was covered with holes and fragments,resulting in the exposure of more fiber bundles and a significant increase in the accessibility of cellulase.These results indicated that the T.sp.SCU31 pretreatment efficiently degraded lignin and disrupted lignocellulosic resistance.

关 键 词：酒糟 栓菌属 微观结构 理化性质 木质素 

分 类 号：TQ35[化学工程]