β-葡聚糖酶高产菌株诱变选育  

Mutation breeding ofβ-glucanase high production strain

作　　者：王克芬 张辉 赵遵明 张强 张法玲 滕来宾 贾仁洁 WANG Kefen;ZHANG Hui;ZHAO Zunming;ZHANG Qiang;ZHANG Faling;TENG Laibing;JIA Renjie(Shandong key laboratory of enzyme fermentation technology of Shandong Long Kete Enzyme Co.,Ltd,Shandong,Linyi 276400,China;Yishui County centre for Animal Disease Control and prevention,Shandong,Linyi 276400,China)

机构地区：[1]山东隆科特酶制剂有限公司山东省酶制剂发酵技术重点实验室,山东临沂276400 [2]沂水县动物疫病预防控制中心,山东临沂276400

出　　处：《畜禽业》2024年第9期11-16,共6页Livestock and Poultry Industry

基　　金：山东省重点研发计划(2021ZDSYS20)。

摘　　要：以木霉(Trichoderma sp.)为出发菌株,通过紫外诱变与光复活交替处理获得了1株高产β-葡聚糖酶突变株UN123。该菌株在摇瓶中的平均酶活大于10000 U/mL,最适反应温度是40℃,90℃条件下保温处理2 h,仍能保持80%以上酶活,最适反应pH是3.0,在pH 2.0~7.0的条件下处理2 h后,相对酶活力保持在80%以上且对胃蛋白酶、胰蛋白酶及凝乳蛋白酶具有良好的耐受性。Using Trichoderma sp.As original strain,a mutant strain UN123 with high yieldβ-glucanase was obtained by UN and photoreactivation.Theβ-glucanase activity of Trichoderma sp.UN123 in shake flask was above 10000 U/Ml,its optimum temperature was 40℃.Theβ-glucanase activity of Trichoderma sp.UN123 maintained above 80%even given heat preservation of 2 h under the temperature of 90℃.Its optimum pH was 3.0.Theβ-glucanase activity of Trichoderma sp.UN123 maintained above 80%under the condition of pH 2.0~7.0 of 2 h and showed god tolerance to pepsin,trypsin,and chymotrypsin.

关 键 词：木霉 Β-葡聚糖酶 紫外诱变 光复活 

分 类 号：S816[农业科学—饲料科学]