基于PI3K/AKT信号通路观察乌梅丸对溃疡性结肠炎大鼠肠黏膜修复作用  

Effect of Wumei pill on intestinal mucosa of ulcerative colitis rats based on PI3K/AKT signal pathway

作　　者：王芳芳[1] 张龙江 周锡晨 李勇骑 王晓雯 WANG Fangfang;ZHANG Longjiang;ZHOU Xichen;LI Yongqi;WANG Xiaowen(The Second Clinical College of Henan University of Traditional Chinese Medicine,Zhengzhou 450002;Second Anorectal Division,Henan Provincial Hospital of Traditional Chinese Medicine,China)

机构地区：[1]河南中医药大学第二临床医学院,河南郑州450002 [2]河南省中医院(河南中医药大学第二附属医院)肛肠科二区

出　　处：《胃肠病学和肝病学杂志》2024年第9期1131-1137,共7页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的基于PI3K/AKT信号通路观察乌梅丸对溃疡性结肠炎(ulcerative colitis,UC)大鼠肠黏膜修复作用。方法采用DSS构建大鼠UC模型,正常对照组为正常大鼠,模型组、乌梅丸组和乌梅丸+740 Y-P组均进行造模,造模后,乌梅丸组按照13.5 g/kg剂量进行灌胃,乌梅丸+740 Y-P组则是在乌梅丸灌胃基础上经腹腔注射50μL的740 Y-P,模型组和正常对照组则给予等剂量的生理盐水,检测各组小鼠的结肠长度,血清中D-LA、LPS及DAO水平,免疫组化检测结肠组织中MUC2和Occludin的表达;Western blotting法检测结肠组织中PI3K/AKT信号通路相关蛋白的表达。结果同时间点,模型组大鼠体质量明显低于正常对照组,乌梅丸组大鼠体质量明显高于模型组和乌梅丸+740 Y-P组(P均<0.05);模型组大鼠的结肠长度明显短于正常对照组,MUC2和Occludin阳性细胞百分比明显低于正常对照组,血清中D-LA、DAO和LPS水平,结肠组织评分,p-PI3K和p-AKT相对表达量高于正常对照组,乌梅丸组大鼠的结肠长度明显长于模型组和乌梅丸+740 Y-P组,MUC2和Occludin阳性细胞百分比、p-PI3K和p-AKT相对表达量明显高于模型组和乌梅丸+740 Y-P组,血清中D-LA、DAO和LPS水平、结肠组织评分低于模型组和乌梅丸+740 Y-P组(P均<0.05)。结论乌梅丸能够减轻结肠组织的病理损伤,改善结肠黏膜的屏障功能,这可能与抑制PI3K/AKT信号通路有关。Objective To observe the repair effect of Wumei pill on intestinal mucosa in rats with ulcerative colitis(UC)based on PI3K/AKT signaling pathway.Methods Rat UC model was established by DSS,and the normal control group were normal rats.The model group,Wumei pill group and Wumei pill+740 Y-P group were all established.After the model was established,Wumei pill group was gavaged with 13.5 g/kg,while Wumei pill+740 Y-P group was intraperitoneally injected with 50μL of 740 Y-P on the basis of gavage.The colon length,serum levels of D-LA,LPS and DAO were detected,and the expressions of MUC2 and Occludin in colon tissue were detected by immunohistochemistry.Western blotting was used to detect the expression of PI3K/AKT signaling pathway related proteins in colon tissue.Results At the same time,the body mass of rats in the model group was significantly lower than that in the normal control group,and the body mass of rats in the Wumei pill group was significantly higher than that in the model group and Wumei pill+740 Y-P group(all P<0.05).The colon length of rats in the model group was significantly shorter than that in the normal control group,and the percentage of MUC2 and Occludin positive cells was significantly lower than that in the normal control group.The levels of D-LA,DAO and LPS in serum,the score of colon tissue,the relative expression of p-PI3K and p-AKT were higher than those in normal control group.The colon length of rats in Wumei pill group was significantly longer than that in model group and Wumei pill+740 Y-P group,and the percentage of positive cells of MUC2 and Occludin,the relative expression of p-PI3K and p-AKT were significantly higher than that in model group and Wumei pill+740 Y-P group.The levels of serum D-LA,DAO and LPS,and the score of colon tissue were lower than those of model group and Wumei pill+740 Y-P group(all P<0.05).Conclusion Wumei pill can alleviate the pathological injury of colon tissue and improve the barrier function of colon mucosa,which may be related to the inhibition of P

关 键 词：PI3K/AKT信号通路 乌梅丸 溃疡性结肠炎 肠黏膜修复 

分 类 号：R574.62[医药卫生—消化系统]