非酒精性脂肪性肝病大鼠肝组织中Smac/DIABLO的表达及其意义  

The expression and significance of Smac/DIABLO in liver tissue of rats with non-alcoholic fatty liver disease

作　　者：陈露 许若思 张彩英 黎慧娟 陈仕[1] CHEN Lu;XU Ruosi;ZHANG Caiying;LI Huijuan;CHEN Shi(Department of Gastroenterology,Brain Hospital of Hunan Province(The Second People′s Hospital of Hunan Province),Changsha 410007,China)

机构地区：[1]湖南省脑科医院(湖南省第二人民医院)消化内科,湖南长沙410007

出　　处：《胃肠病学和肝病学杂志》2024年第9期1179-1184,共6页Chinese Journal of Gastroenterology and Hepatology

基　　金：湖南省教育厅资助项目(21C0220)。

摘　　要：目的探究非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)大鼠肝组织中Smac/DIABLO的表达及其意义。方法将大鼠随机分为对照组、高脂组,对照组采用基础饲料喂养,高脂组采用高脂肪饲料喂养。分别于喂养第4、8、12周观察大鼠体质量和肝脏指数变化,检测血脂指标TG、TC和肝功能指标ALT、AST;HE染色和油红O染色观察肝组织病理变化,流式细胞术检测肝细胞凋亡,免疫组化检测Smac/DIABLO、Caspase-3蛋白表达,Western blotting检测Smac/DIABLO、X染色体连锁凋亡抑制蛋白(X-linked apoptosis inhibitory protein,XIAP)、Caspase-3、Caspase-9蛋白表达,qRT-PCR检测Smac/DIABLO、XIAP、Caspase-3、Caspase-9基因的表达。结果与对照组相比,高脂组大鼠在第4、8、12周肝细胞排列紊乱,有明显的脂肪液泡、炎症细胞浸润、脂质堆积严重,体质量、肝脏指数、TC、TG、ALT、AST、NAS评分、凋亡率显著升高(P<0.05);免疫组化结果显示,相比于对照组,高脂组大鼠肝组织Smac/DIABLO、Caspase-3表达升高,IOD值显著升高(P<0.05);Western blotting和qRT-PCR结果显示,相比于对照组,高脂组大鼠肝组织Smac/DIABLO、Caspase-3、Caspase-9蛋白及mRNA表达显著升高,XIAP表达显著降低(P<0.05)。结论NAFLD大鼠肝组织Smac/DIABLO蛋白表达显著升高,与其肝细胞凋亡密切相关。Objective To investigate the expression and significance of Smac/DIABLO in the liver tissue of rats with non-alcoholic fatty liver disease(NAFLD).[WTHZ]Methods Rats were randomly grouped into a control group and a high-fat group.The control group was fed with basic feed,while the high-fat group was fed with high-fat feed.The changes in body weight and liver index of rats were observed on the 4th,8th and 12th week of feeding,respectively.The kits were applied to detect the blood lipid indicators TG,TC and liver function indicators ALT,AST;HE staining and oil red O staining were applied to observe pathological changes in liver tissue,flow cytometry was applied to detect liver cell apoptosis,immunohistochemistry was applied to detect the expression of Smac/DIABLO and Caspase-3 proteins,Western blotting was applied to detect the expression of Smac/DIABLO,X-linked apoptosis inhibitory protein(XIAP),Caspase-3 and Caspase-9 proteins,qRT-PCR was applied to detect the expression of Smac/DIABLO,XIAP,Caspase-3 and Caspase-9 genes.[WTHZ]Results Compared with the control group,the rats in the high-fat group had disordered arrangement of liver cells at weeks 4,8 and 12,with obvious fat vacuoles,inflammatory cell infiltration,and severe lipid accumulation,the body weight,liver index,TC,TG,ALT,AST,NAS score and apoptosis rate obviously increased(P<0.05);the immunohistochemical results showed that compared to the control group,the expression of Smac/DIABLO and Caspase-3 in the liver tissue of the high-fat group rats increased,the IOD value obviously increased(P<0.05);Western blotting and qRT-PCR results showed that compared to the control group,the expression of Smac/DIABLO,Caspase-3,Caspase-9 proteins and mRNA in the liver tissue of high-fat group rats obviously increased,the expression of XIAP obviously reduced(P<0.05).[WTHZ]Conclusion The expression of Smac/DIABLO protein in liver tissue of NAFLD rats is obviously increased,which is closely related to liver cell apoptosis.

关 键 词：非酒精性脂肪性肝病 SMAC/DIABLO 肝细胞凋亡 

分 类 号：R575[医药卫生—消化系统]