响应面法优化贝莱斯芽孢杆菌XY40-1发酵条件  

Optimization of Fermentation Conditions of Bacillus velezensis XY40-1 by Response Surface Methodology

作　　者：张评美 陶禹 彭宇翔 隆丽 周池 李鑫 蔡雁平[1] ZHANG Ping-mei;TAO Yu;PENG Yu-xiang;LONG Li;ZHOU Chi;LI Xin;CAI Yan-ping(College of Horticulture,Hunan Agricultural University,Changsha 410128,PRC;Hunan Vegetable Research Institute,Changsha 410125,PRC;Hunan Engineering Research Center of Exploration and Utilization of Endophytic Microbial Resources in Plants,Changsha 410125,PRC;Long Ping Branch,Graduate School of Hunan University,Changsha 410125,PRC;Xiangxi National Agricultural Science and Technology Park Management Committee,Xiangxi 416000,PRC)

机构地区：[1]湖南农业大学园艺学院,湖南长沙410128 [2]湖南省蔬菜研究所,湖南长沙410125 [3]植物内生微生物资源挖掘与利用湖南省工程研究中心,湖南长沙410125 [4]湖南大学研究生院隆平分院,湖南长沙410125 [5]湖南湘西国家农业科技园区管理委员会,湖南湘西州416000

出　　处：《湖南农业科学》2024年第9期1-7,共7页Hunan Agricultural Sciences

基　　金：科技援疆、科技援藏项目(2022WK4002);青年科技人才项目(2022RC1047);国家重点研发计划(2022YFD1700100);省重点研发项目(2023NK2015);湖南省农业科技创新资金项目(2022cx115,2022cx39)。

摘　　要：为降低发酵成本,增加贝莱斯芽孢杆菌XY40-1的发酵有效活菌数,采用全因子试验、正交试验、单因素试验结合响应面试验对贝莱斯芽孢杆菌XY40-1的培养体系进行系统优化。结果表明:贝莱斯芽孢杆菌XY40-1最佳发酵培养基配方为葡萄糖0.7%、豆粕1.0%、七水硫酸镁0.5%;XY40-1最佳培养条件为初始pH值7.3、发酵温度36.4℃、转速196.4 r/min、接菌量1.0%,该条件下XY40-1的有效活菌数达到3.57×10^(9)CFU/mL,比优化前提高了92.97%;200 L发酵罐扩大培养的活菌数达到8.23×10^(9)CFU/mL。To reduce the fermentation cost and increase the viable count of Bacillus velezensis XY40-1 in fermentation,this study employed full factorial design,orthogonal design,single factor test,and response surface methodology to optimize the fermentation medium and conditions of this strain.The optimal fermentation medium for XY40-1 was composed of 0.7%glucose,1.0%soybean meal,and 0.5%magnesium sulfate heptahydrate.The fermentation of XY40-1 with an inoculation amount of 1.0%at 36.4℃and 196.4 r/min,and initial pH 7.3 showed the viable count reaching 3.57×10^(9)CFU/mL,which increased by 92.97%compared with that without optimization.The viable count in a 200-L fermenter reached 8.23×10^(9)CFU/mL under the optimized conditions.

关 键 词：贝莱斯芽孢杆菌 有效活菌数 响应面法 发酵优化 培养条件 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]