沙塘鳢肠道致病菌G-2全基因组测序及信息分析  

Whole Genome Sequencing and Bioinformatics Analysis of a Pathogenic Strain G-2 in the Gut of Odontobutis potamophila

作　　者：赵彦华[1,2] 夏爱军 姜虎成[1,2] 薛晖 刘国兴[1,2] ZHAO Yan-hua;XIA Ai-jun;JIANG Hu-cheng;XUE Hui;LIU Guo-xing(Jiangsu Provincial Freshwater Fisheries Research Institute,Nanjing 210017,PRC;Low-Temperature Germplasm Bank of Important Economic Fish of Jiangsu Provincial Science and Technology Resources(Agricultural Germplasm Resources)Coordination Service Platform,Nanjing 210017,PRC)

机构地区：[1]江苏省淡水水产研究所,江苏南京210017 [2]江苏省科技资源(农业种质)统筹服务平台重要经济鱼类低温种质库,江苏南京210017

出　　处：《湖南农业科学》2024年第9期8-14,共7页Hunan Agricultural Sciences

基　　金：江苏现代农业产业技术体系建设专项资金(JATS〔2023〕376,JATS〔2023〕378);江苏省农业重大新品种创制项目(PZCZ201743)。

摘　　要：为探明造成养殖沙塘鳢肠道出血的病原菌G-2的特性,对G-2进行全基因组测序和分析。基因组组分分析和基因功能注释结果显示:G-2的总基因数为5134个,其中蛋白编码基因4977个,非蛋白编码基因157个,非编码基因中tRNA基因107个,rRNA基因42个,sRNA基因8个;分别有5109、3339、3362、2543个基因在对应的Nr、SwissProt、COG、KEGG数据库中获得注释;毒力基因分析表明G-2含有多种肠毒素和溶血素基因、InhA蛋白、磷脂酶及蛋白质毒素,解释了该菌造成养殖沙塘鳢肠道出血的原因。基于G-2菌株的16S rRNA序列信息构建系统进化树,确定其为苏云金芽孢杆菌。Whole genome sequencing and bioinformatics analysis were carried out to characterize the pathogenic strain G-2 causing gut bleeding of cultured Odontobutis potamophila.The results showed that the genome of strain G-2 contained 5134 genes,including 4977 coding genes and 157 non-coding genes(107 tRNA genes,42 rRNA genes,and 8 sRNA genes).In Nr,SwissProt,COG,and KEGG,5109,3339,3362,and 2543 genes,respectively,were annotated.Strain G-2 carried multiple enterotoxin and hemolysin genes and the genes encoding InhA protein,phospholipase,and protein toxins,which explained the gut bleeding caused by this strain.The phylogenetic tree built based on the 16S rRNA gene sequence identified strain G-2 as Bacillus thuringiensis.

关 键 词：沙塘鳢 全基因组测序 苏云金芽孢杆菌 

分 类 号：S943[农业科学—水产养殖]