SIZ1-mediated SUMOylation of CPSF100 promotes plant thermomorphogenesis by controlling alternative polyadenylation  

作　　者：Zhibo Yu Jun Wang Cheng Zhang Qiuna Zhan Leqian Shi Bing Song Danlu Han Jieming Jiang Junwen Huang Xiaolin Ou Zhonghui Zhang Jianbin Lai Qingshun Quinn Li Chengwei Yang 

机构地区：[1]Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,School of Life Science,South China Normal University,Guangzhou 510631,China [2]Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems,Cllege of the Environment and Ecology,Xiamen University,Xiamen,Fujan 361102,China [3]Biomedical Science Division,College of Dental Medicine,Western University of Health Sciences,Pomona,CA 91766,USA

出　　处：《Molecular Plant》2024年第9期1392-1406,共15页分子植物（英文版）

基　　金：supported by grants from the Major Program of Guangdong Basic and Applied Research(2019B030302006);the National Natural Science Foundation of China(32000449,32270292,32270344,32270752,and 32170593);the China Postdoctoral Science Foundation(2020M672674);the Program for Changjang Scholars,the Natural Science Foundation of Guangdong Province,China(2024A1515011497,2020B1515020007,and 2024A1515011071);the Guangdong Provincial Pearl River Talent Plan(2019QN01N108);the National Science Foundation of USA(2347540).

摘　　要：Under warm temperatures,plants adjust their morphologies for environmental adaption via precise gene expression regulation.However,the function and regulation of alternative polyadenylation(APA),an important fine-tuning of gene expression,remains unknown in plant thermomorphogenesis.In this study,we found that SUMOylation,a critical post-translational modification,is induced by a long-term treat-ment at warm temperatures via a SUMO ligase SIZ1 in Arabidopsis.Disruption of SIZ1 altered the global usage of polyadenylation signals and affected the APA dynamic of thermomorphogenesis-related genes.CPSF100,a key subunit of the CPSF complex for polyadenylation regulation,is SUMOylated by SIZ1.Importantly,we demonstrated that SUMOylation is essential for the function of CPSF1oo in genome-wide polyadenylation site choice during thermomorphogenesis.Further analyses revealed that the SUMO conjugation on CPSF100 attenuates its interaction with two isoforms of its partner CPSF30,increasing the nuclear accumulation of CPsF1oo for polyadenylation regulation.In summary,our study uncovers a regulatory mechanism of APA via SiZ1-mediated SUMOylation in plant thermomorpho-genesis.

关 键 词：alternative polyadenylation CPSF100 SiZ1 SUMOYLATION thermomorphogenesis 

分 类 号：Q94[生物学—植物学]