多糖聚电解质静电组装高强度水凝胶膜的组织粘接抑菌性  

Tissue Adhesive Bacteriostasis of Polysaccharide Polyelectrolyte Electrostatic Assembly High Strength Hydrogel Membrane

作　　者：吴强 商伶俐 李学锋 张高文[1,2] 黄以万 龙世军[1,2] WU Qiang;SHANG Lingli;LI Xuefeng;ZHANG Gaowen;HUANG Yiwan;LONG Shijun(School of Materials Science and Engineering,Hubei University of Technology,Wuhan 430068,China;Hubei Provincial Key Laboratory of Green Materials for Light Industry,Hubei University of Technology,Wuhan 430068,China)

机构地区：[1]湖北工业大学材料与化学工程学院,武汉430068 [2]湖北工业大学绿色轻工材料湖北省重点实验室,武汉430068

出　　处：《材料导报》2024年第18期233-238,共6页Materials Reports

基　　金：国家自然科学基金(52073083);湖北隆中实验室自主创新项目(2022ZZ-16)。

摘　　要：由交联的亲水高分子与大量的水组成的水凝胶具有良好的组织相容性,在生物医学领域有广阔的应用前景,但含水率高导致本体强度低(拉伸强度多低于0.1 MPa),表面形成水合层使其很难牢固地粘附在基材的表面。本工作采用两种带相反电荷的多糖聚合物,通过自组装制得了壳聚糖季铵盐/氧化透明质酸(HACC/HA-CHO)聚电解质复合水凝胶膜,带有正电荷及伯氨基团的HACC分子链与带有负电荷及醛基的HA-CHO分子链之间可形成相反电荷间的强静电相互作用,同时伯氨基团与醛基在分子链间还会发生席夫碱反应,两者协同作用使得HACC/HA-CHO水凝胶膜具有优异的拉伸强度(7.67 MPa)。此外,由于新鲜的生物组织表面也富含负电荷与伯氨基团,该水凝胶膜也能与生物组织在界面形成强的相互作用,产生良好的粘接效果。通过进一步引入四(4-羧基苯基)卟啉铁金属簇(Fe-TCPP),得到了Fe-TCPP@HACC/HA-CHO水凝胶膜,其具有优异的抑菌性能(抑菌圈直径为14.8 mm)。本研究为聚电解质复合水凝胶膜在生物医学防污领域中的应用提供了指导。Hydrogels composed of cross-linked hydrophilic polymers and a large amount of water have good biocompatibility and wide application prospects in biomedical fields.However,high water content leads to low bulk strength(tensile strength mostly below 0.1 MPa),and the formation of hydration layer on the surface makes it difficult to firmly adhere to the surface of the substrate.In this work,chitosan quaternary ammonium salt/oxidized hyaluronic acid(HACC/HA-CHO)polyelectrolyte composite hydrogel membrane was prepared by self-assembly using two kinds of polysaccharide polymers with opposite charges.Since the HACC molecular chain with positive charge and primary amino group and HA-CHO molecular chain with negative charge and aldehyde group can form strong electrostatic interaction between opposite charges,and Schiff base reaction can occur between the primary amino group and aldehyde group.The synergistic effect of the two makes the HACC/HA-CHO hydrogel membrane have excellent tensile strength(7.67 MPa).In addition,the fresh biological tissue surface is also rich in negative charges and primary amino groups,and the hydrogel membrane can also form a strong interaction with the biological tissue at the interface and produce a good bonding effect.After introducing 4-carboxyphenyl porphyrin iron metal cluster(Fe-TCPP),Fe-TCPP@HACC/HA-CHO hydrogel membrane was obtained with excellent bacteriostatic performance(the diameter of bacteriostatic zone was 14.8 mm).This work provides guidance for the application of polyelectrolyte composite hydrogel membranes in the field of biomedical antifouling.

关 键 词：聚电解质复合物(PEC) 水凝胶膜 组织粘接 抑菌 多糖 

分 类 号：TB333[一般工业技术—材料科学与工程]