核因子E2相关因子/血红素加氧酶1及血栓素B2T/6-酮-前列腺素F1α信号通路在宫颈癌根治术后发生深静脉血栓大鼠模型中的作用机制  

Mechanism of Nrf2/HO-1 and TXB2/6-Keto-PGF1αsignaling pathway in rat model of deep vein thrombosis after radical cervical cancer surgery

作　　者：莫明静 李得加 张毅[2] 朱桂娟 MO Mingjing;LI Dejia;ZHANG Yi;ZHU Guijuan(Jinzhou Medical University,Jinzhou 121000,China)

机构地区：[1]锦州医科大学,辽宁锦州121000 [2]十堰市人民医院,湖北十堰442000

出　　处：《陕西医学杂志》2024年第10期1330-1334,共5页Shaanxi Medical Journal

基　　金：湖北省教育厅科学技术研究项目计划(B2021168)。

摘　　要：目的:本研究旨在探讨核因子E2相关因子(Nrf2)/血红素加氧酶1(HO-1)及血栓素B2(TXB2)/6-酮-前列腺素F1α(6-Keto-PGF1α)信号通路在宫颈癌根治术后发生深静脉血栓大鼠模型中的作用机制。方法:购买60只4~6周龄裸鼠,体重(180±20)g,随机将60只大鼠分为空白对照组、模型组及深静脉血栓组各20只。其中空白组正常饲养1周;模型组及深静脉血栓组均建立宫颈癌动物模型,模型组造模成功后,正常饲养;深静脉血栓组给予宫颈癌根治术治疗,正常饲养。观察宫颈癌根治术后发生深静脉血栓HE染色切片,原位末端标记法(TUNEL)检测各组大鼠血管组织内皮细胞凋亡情况,全自动凝血分析仪检测各组大鼠凝血指标,酶联免疫吸附法测定TXB2/6-Keto-PGF1α水平,免疫印迹检测Nrf2/HO-1信号通路。结果:实验过程中,三组大鼠均未出现意外死亡,存活率均为100%。模型组未形成血栓,深静脉血栓组造模后1、3、7、14 d成栓率依次为50.00%(10/20)、95.00%(19/20)、100%(20/20)。大鼠下腔静脉产生的血栓尾部为红色血栓、中间为混合血栓、头部为白色血栓。深静脉血栓组血管内皮凋亡率高于模型组(P<0.05)。深静脉血栓组凝血酶时间、凝血酶原时间、活化部分凝血活酶时间低于模型组及空白对照组,D-二聚体高于模型组及空白对照组(均P<0.05);模型组凝血酶时间、凝血酶原时间、活化部分凝血活酶时间低于空白对照组,D-二聚体高于空白对照组(均P<0.05)。深静脉血栓组TXB2、TXB2/6-Keto-PGF1α高于模型组及空白对照组,6-Keto-PGF1α低于模型组及空白对照组(均P<0.05);模型组TXB2、TXB2/6-Keto-PGF1α高于空白对照组,6-Keto-PGF1α低于空白对照组(均P<0.05)。深静脉血栓组Nrf2、HO-1低于模型组及空白对照组,模型组Nrf2/HO-1低于空白对照组(均P<0.05)。结论:TXB2/6-Keto-PGF1α、Nrf2/HO-1信号通路在宫颈癌根治术后深静脉血栓大鼠中异常表达,也是宫颈癌根�Objective:To investigate the mechanism of nuclear factor E2-associated factor-2(Nrf2)/heme oxygenase-1(HO-1)and thromboxane B2(TXB2)/6-keto-PGF1-α(6-Keto-PGF1α)signaling pathways in rats with deep vein thrombosis after radical cervical cancer surgery.Methods:A total of 60 nude mice aged 4 to 6 weeks with body weight of(180±20)g were purchased and randomly divided into blank control group,model group and deep vein thrombosis(DVT)group with 20 rats each.The blank group was fed normally for 1 week.Cervical cancer animal models were established in model group and deep vein thrombosis group,and the blank control group was fed normally.After the model group was successfully molded,fed normally.The deep vein thrombosis group was treated with radical resection of cervical cancer and fed normally.Deep vein thrombosis after radical cervical cancer resection was observed.The apoptosis of vascular endothelial cells in each group was detected by TUNEL method,the coagulation indexes was detected by automatic coagulation analyzer,and the level of TXB2/6-Keto-PGF1αwas determined by enzymed-linked immunosorbent assay.The Nrf2/HO-1 signaling pathway was detected by Western blot.Results:During the experiment,no accidental death occurred in the three groups of rats,and the survival rate was 100%.There was no thrombosis in the model group.The thrombus formation rate was 50.00%(10/20),95.00%(19/20)and 100%(20/20)in the DVT group at 1,3,7 and 14 days,respectively.The thrombus produced by inferior vena cava in rats was red in the tail,mixed in the middle,and white in the head.The apoptosis rate of vascular endothelium in DVT group was higher than that in model group(P<0.05).Thrombin time,prothrombin time and activated partial thrombin time in DVT group were lower than those in model group and blank control group,and D-dimer was higher than those in model group and blank control group(all P<0.05).The thrombin time,prothrombin time and activated partial thrombin time in model group were lower than those in blank control group,and the

关 键 词：核因子E2相关因子/血红素加氧酶1 血栓素B2T/6-酮-前列腺素F1α 宫颈癌根治术后 深静脉血栓大鼠 机制 

分 类 号：R737.33[医药卫生—肿瘤]