负载RGD的PLA-HA骨仿生支架促进骨缺损早期修复的研究  

RGD-loaded PLA-HA bone biomimetic scaffold for promoting early bone defect repair

作　　者：李欣玲 赵雨晴[1,2] 韩金煜 韩晶媛 吴凡 王菁 LI Xin-ling;ZHAO Yu-qing;HAN Jin-yu;HAN Jing-yuan;WU Fan;WANG Jing(Affiliated Stomatological Hospital of Jiamusi University,Jiamusi 154002,China;不详)

机构地区：[1]佳木斯大学附属口腔医院,黑龙江佳木斯154002 [2]口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔生物工程工程技术中心,空军军医大学第三附属医院种植科,陕西西安710032

出　　处：《牙体牙髓牙周病学杂志》2024年第6期320-324,共5页Chinese Journal of Conservative Dentistry

摘　　要：目的:使用精氨酸-甘氨酸-天冬氨酸肽(RGD)修饰骨仿生支架并探究其应用于大鼠股骨缺损区的成骨性能。方法:通过三维(3D)打印技术制备聚乳酸-羟基磷灰石支架(PLA-HA),在其表面修饰甲基丙烯酰化明胶(GelMA)和RGD构建复合水凝胶支架PLA-HA/GelMA/RGD(PHD),扫描电镜(SEM)表征支架表面形貌,CCK-8实验评估骨髓间充质干细胞(BMSCs)增殖能力。将18只SD大鼠随机均分为3组(n=6)制备股骨缺损模型,分别植入PLA-HA/GelMA(PHG)、PHD支架,空白对照组不植入。于术后4周拍摄Micro-CT测算骨缺损面积和新生骨指标,并对缺损区的股骨样本进行HE染色和Masson三色染色观察组织学形态。结果:SEM观察到支架和水凝胶孔径明显,同时水凝胶稳健的连接到支架表面;CCK-8结果显示与PHG组相比,PHD组显示细胞增殖活性更佳。术后4周,Micro-CT三维重建图像显示空白组和PHG组新骨形成较少,PHD组骨缺损内部见大量新骨形成,同时定量分析发现该组骨体积分数、骨小梁厚度均显著大于空白组和PHG组。组织学染色结果显示PHD组形成连续且致密的骨组织。结论:负载RGD的PLA-HA骨仿生支架在体外具有促进成骨细胞增殖的能力,同时在体内诱导大鼠股骨缺损区新骨形成,成功地实现了早期骨缺损修复。AIM:To investigate osteogenic properties of scaffolds in rat femoral defects by modifying biomimetic scaffolds with the Arginine-Glycine-Aspartic acid(RGD).METHODS:GelMA and RGD were modified in poly(lactic acid)-hydroxyapatite(PLA-HA)scaffolds prepared by three-dimensional(3D)printing to construct composite hydrogel scaffolds PLA-HA/GelMA/RGD(PHD).The surface morphology of PHD scaffold was observed by scanning electron microscope(SEM),and the proliferation of bone marrow mesenchymal stem cells(BMSCs)was assessed by CCK-8 assay.18 SD rats were randomly divided into three groups(n=6)and implanted with PLA-HA/GelMA(PHG)and PHD scaffolds,while no implantation was performed in the blank control group.At 4 weeks,the bone defect area and new bone index were measured by micro-CT,and the femoral samples in the defect area were stained with H&E staining as well as Masson trichrome staining to observe the histological morphology.RESULTS:SEM images indicated that the pore sizes of the scaffolds were distinct,and GelMA was stably attached to the surface of the scaffolds.CCK-8 results demonstrated the cell proliferation in the PHD group was superior compared to PHG group.At 4 weeks after surgery,the 3D reconstructed micro-CT images revealed that abundant new bone was observed in the PHD group compared to the blank group and PHG group.Simultaneously,quantitative analysis indicated bone volume fraction and bone trabecular thickness were significantly enhanced in PHD group.Histological staining showed that the PHD group formed continuous and compact bone tissue.CONCLUSION:The RGD-loaded PLA-HA bone biomimetic scaffolds can facilitate osteoblast proliferation in vitro and induce new bone formation in the rat femur defect model,thereby successfully achieving early bone defect repair.

关 键 词：聚乳酸-羟基磷灰石支架 甲基丙烯酰化明胶 精氨酸-甘氨酸-天冬氨酸肽 骨再生 

分 类 号：R78[医药卫生—口腔医学]