红树老鼠簕内生真菌Aspergillus terreus GXIMD 03158次级代谢产物研究  

Studies on secondary metabolites of endophytic fungus Aspergillus terreus GXIMD 03158 isolated from mangroves Acanthus ilicifolius L.

作　　者：安凡 姜悦 王宇 曹广萍 高程海 刘永宏 易湘茜 白猛 AN Fan;JIANG Yue;WANG Yu;CAO Guangping;GAO Chenghai;LIU Yonghong;YI Xiangxi;BAI Meng(Institute of Marine Drugs/Faculty of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China;Guangxi Key Laboratory of Marine Drugs,Nanning 530200,China)

机构地区：[1]广西中医药大学海洋药物研究院/药学院,广西南宁530200 [2]广西海洋药物重点实验室,广西南宁530200

出　　处：《热带海洋学报》2024年第5期41-48,共8页Journal of Tropical Oceanography

基　　金：广西自然科学基金项目(2023GXNSFAA026313、2021GXNSFBA220072、2020GXNSFGA297002、2021GXNSFDA075010);广西中医药大学2020年博士启动基金项目(2020BS024);广西八桂学者专项基金项目(05019055);广西中医药大学“桂派中医药传承创新团队”项目(2022A007)。

摘　　要：文章对红树老鼠簕(Acanthus ilicifolius L.)内生真菌Aspergillus terreus GXIMD 03158的抑菌活性次级代谢产物进行研究。采用多种色谱技术对该菌次级代谢产物进行分离纯化,结合核磁氢谱、碳谱和高分辨率质谱数据综合解析鉴定单体化合物结构。从A.terreus GXIMD 03158代谢产物中共分离出10个化合物,经鉴定分别为dankasterones A(1),(14α,22E)-14-hydroxyergosta-4,7,22-triene-3,6-dione(2),steresterone B(3),ergosta-4,6,8(14),22-tetraen-3-one(4),herbarulide(5),demethylincisterol A3(6),isochaetochromin B1(7),isochaetochromin B2(8),(E)-7,9-二烯-11-羰基硬脂酸(9)和aspergilfuranone A(10)。并采用微量肉汤稀释法检测所有化合物对白色葡萄球菌(Staphylococcus albus)、金黄色葡萄球菌(Staphylococcus aureus)、表皮葡萄球菌(Staphylococcus epidermidis)、耐甲氧西林金黄色葡萄球菌(Methicillin-resistant Staphylococcus aureus,MRSA)和副溶血弧菌(Vibrio parahaemolyticus)的最低抑菌浓度(minimal inhibitory concentrations,MIC),结果显示化合物6对S.albus、S.aureus和S.epidermidis的MIC分别为3.12、6.25和3.12μg·mL^(−1);化合物9对S.albus和S.aureus的MIC分别为1.56和3.12μg·mL^(−1)。This work studies the secondary metabolites with antibacterial activity from Aspergillus terreus GXIMD 03158,an endophytic fungus isolated from mangroves Acanthus ilicifolius L.The secondary metabolites of the strains were separated and purified by various chromatographic techniques,and the structures of monomer compounds were identified by comprehensive analyses of 1H,13C NMR and HREIMS date.Ten compounds were isolated from the metabolites of A.terreus GXIMD 03158,and identified as dankasterones A(1),(14α,22E)-14-hydroxyergosta-4,7,22-triene-3,6-dione(2),steresterone B(3),ergosta-4,6,8(14),22-tetraen-3-one(4),herbarulide(5),demethylincisterol A3(6),isochaetochromin B1(7),isochaetochromin B2(8),(E)-7,9-diene-11-carbonyl stearic acid(9)and aspergilfuranone A(10).The minimal inhibitory concentrations(MIC)of all compounds against Staphylococcus albus,Staphylococcus aureus,Staphylococcus epidermidis,Methicillin-resistant Staphylococcus aureus and Vibrio parahaemolyticus were determined by broth microdilution method,the results showed that compound 6 exhibited significant antibacterial activity against S.albus,S.aureus and S.epidermidis,and the MIC values were 3.12,6.25 and 3.12μg·mL^(−1),respectively;compound 9 exhibited significant antibacterial activity against S.albus and S.aureus,and the MIC values were 1.56 and 3.12μg·mL^(−1),respectively.This study provides a theoretical basis for the research on development and utilization of mangrove Acanthus ilicifolius L.and its endophytic fungi.

关 键 词：红树老鼠簕 内生真菌 曲霉属真菌 次级代谢产物 抑菌活性 

分 类 号：P735.51[天文地球—海洋生物学]