高效液相色谱指纹图谱结合多元数据分析用于佛手与枸橼的鉴别  

Identification of Citri Sarcodactylis Fructus and Citri Fructus based on HPLC fingerprint coupled with multivariate data analysis

作　　者：吴笛 蔺晓源[1,2] 刘凯[1,2] 陈宇航[4] WU Di;LIN Xiaoyuan;LIU Kai;CHEN Yuhang(The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;Key Laboratory of Research on Prevention and Treatment of Major Diseases in Chinese Internal Medicine and Transformation of Achievements of Ministry of Education,Changsha 410007,China;Hunan Provincial University Key Laboratory of the Regional Characteristic Traditional Chinese Medicine Resources and Ecological Agriculture,Changsha 410219,China;School of pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学第一附属医院,长沙410007 [2]中医内科重大疾病防治研究及转化教育部重点实验室,长沙410007 [3]区域特色中药资源与生态农业湖南省普通高等学校重点实验室,长沙410219 [4]湖南中医药大学药学院,长沙410208

出　　处：《中国药学杂志》2024年第16期1504-1512,共9页Chinese Pharmaceutical Journal

基　　金：湖南省中医药科研重点指导课题资助(C2023009)。

摘　　要：目的 探讨采用高效液相色谱指纹图谱结合多元数据分析对佛手与枸橼进行品种鉴别的可行性。方法 采用高效液相色谱法分别建立佛手与枸橼的指纹图谱,采用“中药色谱指纹图谱相似度评价系统(2004A版)”分别对12批佛手、10批枸橼进行相似度评价,并结合系统聚类分析、主成分分析、正交偏最小二乘判别分析进行考察。结果 分别建立了佛手与枸橼的高效液相色谱指纹图谱,12批佛手、10批枸橼与各自对照指纹图谱的相似度均>0.9,12批佛手与枸橼对照指纹图谱的相似度、10批枸橼与佛手对照指纹图谱的相似度均<0.9,表明佛手与枸橼的化学成分存在显著的差异;系统聚类分析、主成分分析和正交偏最小二乘判别分析均将22批样品分为两类,与品种区分一致;正交偏最小二乘判别分析筛选得到3个差异标志物,经与对照品比对,确认其中2个差异性成分分别为5,7-二甲氧基香豆素、橙皮苷;统计分析提示根据橙皮苷与5,7-二甲氧基香豆素的绝对峰面积的比值可简便而准确地区分佛手与枸橼。结论 高效液相色谱指纹图谱结合多元数据分析可用于佛手与枸橼的鉴别。OBJECTIVE To investigate the feasibility of performing identification of Citri Sarcodactylis Fructus(CSF) and Citri Fructus(CF) based on high performance liquid chromatography(HPLC) fingerprint coupled with multivariate data analysis.METHODS HPLC method was applied to establish the fingerprints of CSF and CF.Similarity evaluation of 12 batches of CSF samples and 10 batches of CF samples was performed by Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2004A edition).Hierarchical cluster analysis(HCA),principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA) were used for data analysis.RESULTS HPLC fingerprints of CSF and CF were established,respectively.The fingerprint similarities of CSF samples,as well as the fingerprint similarities of CF samples,were all greater than 0.9.The similarities between the fingerprints of CSF samples and the control fingerprint of CF,as well as the similarities between the fingerprints of CF samples and the control fingerprint of CSF,were all less than 0.9,which indicated significant differences in chemical composition between CSF and CF.The total 22 samples were divided into two groups by HCA,PCA and OPLS-DA,which was consistent with the two varieties.Three differential markers were screened by OPLS-DA and two of them were identified as 5,7-dimethoxycoumarin and hesperidin by reference substances.The statistical analysis indicated that the ratio of absolute peak area(APA) of hesperidin and 5,7-dimethoxycoumarin can distinguish CSF and CF from each other simply and accurately.CONCLUSION HPLC fingerprint coupled with multivariate data analysis can be used to identify CSF and CF.

关 键 词：佛手 枸橼 高效液相色谱指纹图谱 系统聚类分析 主成分分析 正交偏最小二乘判别分析 品种鉴别 

分 类 号：R284.1[医药卫生—中药学]