双波段LED照明的基因测序荧光显微系统设计  

Design of gene sequencing fluorescence microscopic system with dual-band LED illumination

作　　者：刘美辰 孟庆宇[1] 张洪文[1] 王克军[1] 李云辉[1] 王思琦 王江南 LIU Meichen;MENG Qingyu;ZHANG Hongwen;WANG Kejun;LI Yunhui;WANG Siqi;WANG Jiangnan(Changchun Institute of Optics,Fine Mechanics and Physics,Chinese Academy of Sciences,Changchun 130033,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院长春光学精密机械与物理研究所,吉林长春130033 [2]中国科学院大学,北京100049

出　　处：《光学精密工程》2024年第16期2455-2463,共9页Optics and Precision Engineering

基　　金：国家自然科学基金面上项目(No.62375264);中国科学院青年创新促进会优秀会员(No.Y2023061)。

摘　　要：荧光显微成像系统是基因测序仪的关键模块之一,为了确保基因测序的准确性,荧光成像系统要能够实现高分辨率,高质量成像。为满足对基因测序仪小型化,低成本,高分辨率的需求,本文设计了一种基于窄带LED照明的双波段基因测序荧光显微成像系统。首先,根据荧光显微成像的基本原理,利用临界照明原理设计基于LED的照明系统,通过仿真分析优化照明均匀性,并利用分束镜完成高分辨率、高成像质量的成像系统设计。通过设计方案完成了样机研制,并验证了成像性能。根据测试结果显示,所设计的显微系统XY方向分辨率为0.8μm,照明均匀性优于80%,满足高分辨率成像要求。相较其他系统而言,该系统整机具有小型化,低成本的特点,满足基因测序需求,在发展小型化基因测序仪领域具有重要应用前景。The fluorescence microscopy imaging system is one of the key modules of the gene sequencer.In order to ensure the accuracy of gene sequencing,the fluorescence imaging system must be able to achieve high resolution and high-quality imaging.To meet the requirements of miniaturization,low cost,and high resolution for gene sequencers,this paper designed a dual band gene sequencing fluorescence microscopy imaging system based on narrowband LED illumination.Firstly,based on the basic principle of fluorescence microscopy imaging,an LED based illumination system was designed using the critical illumination principle.The illumination uniformity was optimized through simulation analysis,and a high-resolution and high imaging quality imaging system was designed using a beam splitter.The prototype development was completed through the design scheme,and the imaging performance was verified.According to the test results,the designed microscopy system has a resolution of 0.8μm in the XY direction.The illumination uniformity is better than 80%,meeting the requirements of high-resolution imaging.Compared with other systems,this system has the characteristics of miniaturization and low cost,meeting the needs of gene sequencing,and has important application prospects in the development of miniaturized gene sequencers.

关 键 词：基因测序 LED照明 荧光成像 光学系统 

分 类 号：TP394.1[自动化与计算机技术—计算机应用技术] TH691.9[自动化与计算机技术—计算机科学与技术]