不同品系及苯丙氨酸饲喂对紫萍中类黄酮累积的影响  

Effects of different strains and phenylalanine supplementation on accumulation of flavonoids in Spirodela polyrhiza(L.)Schleid.

作　　者：王易 王文静 向蓓蓓[1] WANG Yi;WANG Wenjing;XIANG Beibei(School of Chinese Materia Medica,Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]天津中医药大学中药学院,天津301617

出　　处：《药学研究》2024年第9期846-853,共8页Journal of Pharmaceutical Research

基　　金：国家自然科学基金项目(No.81303303、82074120)。

摘　　要：目的探讨不同品系紫萍主要类黄酮含量以及苯丙氨酸饲喂对不同品系紫萍生长及总黄酮含量的影响。方法对天津、武汉、贵阳采集的浮萍样本进行形态学及DNA条形码鉴定,利用超高效液相色谱(UPLC)测定6个品系紫萍的木犀草苷、荭草苷和牡荆素含量。用0、0.005、0.05、0.5、1 mmol·L^(-1)浓度的苯丙氨酸饲喂不同品系紫萍10 d,收样测定其鲜重、干重及总黄酮含量。结果采集的浮萍样本经鉴定为紫萍属。6个品系紫萍中p143的总黄酮和3种主要类黄酮的含量最高。0.005 mmol·L^(-1)苯丙氨酸饲喂明显促进WHD和5543的鲜重累积。0.05 mmol·L^(-1)苯丙氨酸饲喂分别使5543、W0716、9509的总黄酮含量上调了16.5%、33.1%和42.6%。结论6个品系紫萍中p143的黄酮含量最高,低浓度苯丙氨酸饲喂可以促进部分品系紫萍的生长及总黄酮含量累积。Objective To explore the main flavonoid constituents across various strains of Spirodela polyrhiza(L.)Schleid.,and to assess the impact of phenylalanine supplementation on the growth and total flavonoid yield of these strains,the following investigation was conducted.Methods Morphological and DNA barcoding identification were performed on duckweed samples collected from Tianjin,Wuhan,and Guiyang.Luteolin,orientin and vitexin levels in six strains were quantified using UPLC.Different S.polyrrhiza strains were cultivated with 0,0.005,0.05,0.5 and 1 mmol·L^(-1) phenylalanine for 10 days,followed by measurement of fresh weight,dry weight and total flavonoid contents.Results The collected duckweed samples were identified as Spirodela.Strain p143 exhibited the highest total flavonoids and three main flavonoids contents among the six strains.Phenylalanine supplementation at 0.005 mmol·L^(-1) significantly enhanced fresh weight accumulation in strains WHD and 5543.Treatment with 0.05 mmol·L^(-1) phenylalanine increased the total flavonoid content by 16.5%,33.1%and 42.6%in strains 5543,W0716 and 9509,respectively.Conclusion Strain p143 exhibited the highest flavonoid content among the six strains.Low-dose phenylalanine supplementation can promote the growth and total flavonoid contents accumulation of some strains of S.polyrrhiza.

关 键 词：紫萍 类黄酮 苯丙氨酸 超高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]