结肠癌患者肿瘤浸润淋巴细胞表型与临床病理特征和肠道菌群的关系研究  

Study on the relationship between phenotype of tumor-infiltrating lymphocytes and clinicopathological characteristics and gut microbiota in patients with colon cancer

作　　者：黄超 方兴刚 陈璐 Huang Chao;Fang Xinggang;Chen Lu(Department of Integrated Traditional Chinese and Western Medicine,Taihe Hospital(Affiliated Hospital of Hubei University of Medicine),Shiyan 442000,Hubei,China)

机构地区：[1]十堰市太和医院(湖北医药学院附属医院)中西医结合科,湖北十堰442000

出　　处：《肿瘤代谢与营养电子杂志》2024年第4期547-552,共6页Electronic Journal of Metabolism and Nutrition of Cancer

基　　金：2022年度湖北省教育厅科学研究计划指导性项目(B2022131)。

摘　　要：目的探究结肠癌患者肿瘤浸润淋巴细胞(TIL)表型与临床病理特征和肠道菌群的关系。方法选取2017年6月至2023年2月湖北省十堰市太和医院收治的76例结肠癌患者为结肠癌组,并选择同期体检的76例健康人为对照组。检测两组受试者TIL表型表达水平,荧光定量PCR检测肠道菌群,收集结肠癌患者临床病理资料。采用Spearman分析TIL表型与肠道菌群的相关性。结果与对照组相比,结肠癌组CD4^(+)T细胞水平显著升高[(28.05±5.48)%比(32.49±6.13)%],CD3^(+)T细胞、CD8^(+)T细胞水平显著降低[(60.47±6.53)%比(53.17±6.37)%]、[(30.69±6.38)%比(24.85±6.42)%](P<0.05)。根据结肠癌患者CD3^(+)T细胞(52.62%)、CD8^(+)T细胞(25.87%)、CD4^(+)T细胞(31.94%)的中位值,将其分为CD3^(+)T细胞高表达38例,CD3^(+)T细胞低表达38例,CD8^(+)T细胞高表达38例,CD8^(+)T细胞低表达38例,CD4^(+)T细胞高表达38例,CD4^(+)T细胞低表达38例。有淋巴结转移、肿瘤分化程度越高、TNM分期越高,在CD3^(+)T细胞、CD8^(+)T细胞、CD4^(+)T细胞高表达、低表达患者间差异有统计学意义(χ2=5.330、4.659、6.786;χ2=7.962、10.483、4.343;χ2=11.120、7.280、13.300;P<0.05)。与对照组相比,结肠癌组脆弱拟杆菌、大肠埃希菌、肠球菌数量显著增加[(6.58±0.83)lgN/g比(8.59±1.36)lgN/g]、[(7.36±1.04)lgN/g比(9.73±1.25)lgN/g]、[(7.21±1.08)lgN/g比(9.28±1.74)lgN/g],乳酸杆菌、双歧杆菌数量显著下降[(8.85±1.46)lgN/g比(6.34±1.22)lgN/g]、[(8.94±1.09)lgN/g比(6.17±1.32)lgN/g](P<0.05)。Spearman相关分析结果显示,结肠癌患者脆弱拟杆菌、大肠埃希菌、肠球菌与CD3^(+)、CD8^(+)T细胞占比呈负相关(r=-0.482、-0.459、-0.532,P<0.05;r=-0.438、-0.472、-0.508,P<0.05),乳酸杆菌、双歧杆菌与CD3^(+)、CD8^(+)T细胞占比呈正相关(r=0.581、0.507,P<0.05;r=0.536、0.467,P<0.05),脆弱拟杆菌、大肠埃希菌、肠球菌与CD4^(+)T细胞占比呈正相关(r=0.483、0.495、0.461,P<0.05),乳酸杆菌Objective To explore the relationship between the phenotype of tumor-infiltrating lymphocytes(TIL)and clinicopathological characteristics and gut microbiota in patients with colon cancer.Method From June 2017 to February 2023,76 colon cancer patients admitted to Taihe Hospital were collected as the colon cancer group,and 76 healthy individuals who underwent physical examinations during the same period were collected as the control group.The TIL phenotype expression levels of two groups were detected,fluorescent quantitative PCR was applied to detect gut microbiota,and the clinical and pathological data of colon cancer patients were collected.Spearman was applied to analyze the correlation between TIL phenotype and gut microbiota.Result Compared with the control group,the level of CD4^(+)T cell in the colon cancer group was obviously increased[(28.05±5.48)%vs(32.49±6.13)%],while the levels of CD3^(+)T cell and CD8^(+)T cell were obviously reduced[(60.47±6.53)%vs(53.17±6.37)%],[(30.69±6.38)%vs(24.85±6.42)%](P<0.05).According to the median values of CD3^(+)T cell(52.62%),CD8^(+)T cell(25.87%)and CD4^(+)T cell(31.94%),colon cancer patients were divided into 38 cases with high expression of CD3^(+)T cell,38 cases with low expression of CD3^(+)T cell,38 cases with high expression of CD8^(+)T cell,38 cases with low expression of CD8^(+)T cell,38 cases with high expression of CD4^(+),T cell and 38 cases with low expression of CD4^(+)T cell.There were lymph node metastasis,the higher the degree of tumor differentiation,the higher the TNM stage,and the difference between high and low expression of CD3^(+)T cell,CD8^(+)T cell and CD4^(+)T cell was statistically significant(χ2=5.330,4.659,6.786;χ2=7.962,10.483,4.343;χ2=11.120,7.280,13.300,P<0.05).Compared with the control group,the numbers of Bacteroides fragilis,Escherichia coli and Enterococcus in the colon cancer group increased obviously[(6.58±0.83)lgN/g vs(8.59±1.36)lgN/g],[(7.36±1.04)lgN/g vs(9.73±1.25)lgN/g],[(7.21±1.08)lgN/g vs(9.28±1.74)lgN/g],while t

关 键 词：结肠癌 肿瘤浸润淋巴细胞 临床病理特征 肠道菌群 免疫 CD3^(+)T细胞 CD4^(+)T细胞 CD8^(+)T细胞 

分 类 号：R735.35[医药卫生—肿瘤]