免疫组化及荧光原位杂交检测人类表皮生长因子受体2的临床意义  

Clinical significance of immunohistochemistry and fluorescence in situ hybridization for detection of human epidermal growth factor receptor 2

作　　者：李慧明 徐芳华 袁敏 张志勤 LI Huiming;XU Fanghua;YUAN Min;ZHANG Zhiqin(Pingxiang People's Hosptial,Pingxiang 337000,China)

机构地区：[1]萍乡市人民医院,江西萍乡337000

出　　处：《临床医药实践》2024年第10期754-758,共5页Proceeding of Clinical Medicine

基　　金：江西省卫生健康委员会科技计划项目(项目编号:202212137)。

摘　　要：目的:探讨结直肠癌中不同免疫组化评分标准下人类表皮生长因子受体2(HER2)蛋白表达情况及荧光原位杂交方法检测HER2基因扩增与临床病理特征之间的关系。方法:选择2019年1月-2021年12月结直肠癌根治术的石蜡组织标本100例,同时收集患者的临床和病理信息。采用三种不同评分标准对所有病例行全自动免疫组化(IHC)检测HER2蛋白和荧光原位杂交(FISH)检测HER2基因表达,分析其与临床病理特征的关系。结果:100例结直肠癌组织中,三种不同评分标准下HER2的蛋白阳性表达率分别为34%,27%和20%,差异无统计学意义(P>0.05)。IHC评分标准1下HER2蛋白表达3+,2+,1+,0+与基因扩增的一致性分别为50.0%,12.5%,2.9%和0。IHC评分标准2下HER2蛋白表达与基因扩增的一致性分别为71.4%,15.0%,2.9%和0,IHC评分标准3下HER2蛋白表达与基因扩增的一致性分别为100.0%,20.0%,2.9%和0,IHC评分标准3下HER2蛋白与基因扩增一致性最高。FISH检测HER2基因扩增9例(9.0%)。HER2基因扩增与年龄、性别、病理分期、浸润深度无相关性(P>0.05),与分化程度、淋巴结转移有相关性(P<0.05)。结论:三种评分标准检测HER2蛋白表达阳性率比较,差异无统计学意义,但评分标准3下HER2蛋白表达与基因扩增的一致性最高。HER2基因扩增与分化程度和淋巴结转移有关。临床上应优先选用评分标准3进行HER2蛋白表达检测。Objective:To investigate the relationship between human epidermal growth factor receptor 2(HER2)gene status distribution,HER2 gene amplification/HER2 protein expression and clinicopathological features in colorectal cancer according to the differentstandard for evaluation.Methods:Paraffin tissue samples of 100 patients undergoing radical resection of colorectal cancer in PingXiang people's Hospital from January 2019 to December 2021 were collected,and the clinical and pathological information of patients were collected.Automatic immunohistochemistry(IHC)was used to detect HER2 protein and fluorescence in situ hybridization(FISH)was used to detect HER2 gene expression in all cases,and its relationship with clinicopathological features was analyzed.Results:In 100 patients with colorectal cancer group,the positive expression rates of HER2 protein were 34%,27%and 20%under three different scoring criteria respectively.The difference was no statistical significant(P>0.05).The consistency of HER2 protein expression of 3+,2+,1+,0+and gene amplification under IHC scoring criterion 1 was 50.0%,12.5%,2.6%,0,respectively.The consistency of HER2 protein expression and gene amplification under IHC score 2 was 71.4%,15.0%,2.9%,0,respectively;the consistency of HER2 protein expression and gene amplification under IHC score 3 was 100.0%,20.0%,2.9%,0,respectively.The consistency of HER2 protein and gene amplification was the highest under IHC scoring criterion 3.HER2 gene amplification was detected by FISH in 9 cases(9.0%).HER2 gene amplification was not correlated with age,sex,pathologic stage,and depth of invasion(P>0.05),but was correlated with differentiation degree and lymph node metastasis(P<0.05).Conclusion:There was no statistical difference in the positive rate of HER2 protein expression among the three scoring criteria,but the consistency of HER2 protein expression and gene amplification was the highest under scoring criteria 3.The amplification of HER2 gene was related to the degree of differentiation and lymph node met

关 键 词：免疫组化 荧光原位杂交 结直肠癌 人类表皮生长因子受体2 

分 类 号：R735[医药卫生—肿瘤]