野生香菇母种纯化与保藏培养基筛选  

Culture Selection of Purification and Preservation of Mother Spawn From Wild Lentinula edodes

作　　者：王旭[1] 王鑫[1] 胡志强[1] 杜忠伟[1] 李健 朴姬妍 孙秀霞 武文琳 Wang Xu;Wang Xin;Hu Zhiqiang;Du Zhongwei;Li Jian;Piao Jiyan;Sun Xiuxia;Wu Wenlin(Yanbian Academy of Agricultural Sciences,Yanji,Jilin 133000,China)

机构地区：[1]延边朝鲜族自治州农业科学院,吉林延吉133000

出　　处：《东南园艺》2024年第4期308-313,共6页Southeast Horticulture

基　　金：财政部和农业农村部国家现代农业产业技术体系资助项目(CARS-20);吉林省科技发展项目(20220404010NC);吉林省农业关键核心技术示范推广项目(202300601);延边州科技发展项目(2022NS15)。

摘　　要：【目的】筛选野生香菇的母种纯化与保藏培养基,为培养和保藏野生香菇菌株种质资源提供适宜条件。【方法】将从吉林省安图县采集到的野生香菇菌株培养成母种,再分别设置4种纯化与保藏培养基配方,比较培养后菌丝的生长情况与菌落的颜色差异,筛选适宜的培养基配方。【结果】母种纯化培养基中以配方四(马铃薯200 g、木屑10 g、黄豆粉10 g、葡萄糖20 g、琼脂20 g、水1 L)表现最优,其菌丝在第3 d开始萌发,菌丝生长速度最快,达0.51 cm/d,长势旺盛、白色,菌落无色素。母种保藏培养基中以配方A(细木屑78%、麦麸20%、石灰1%、石膏1%)的表现好,其菌丝萌发率高,长势旺盛且整齐,菌落无色素,能保藏15个月。【结论】通过研究筛选出野生香菇适宜的母种纯化与保藏培养基,为香菇野生资源的收集纯化与长期保藏奠定了基础。【Objective】In order to select the culture to purificate and preservate of wild Lentinula edodes,to provide suitable conditions for cultivating and preserving germplasm resources of wild Shiitake strains.【Method】Cultured from strain comes to spawn of Shiitake which collected from Antu County,Jilin Province,set up four kinds of purification and preservation medium,compared the difference of growth of mycelium and the colorof the colonies.【Result】Both hyphal may germinate in different purificated and preserve cultures of the spawn of L.edodes,but has big differences.The results showed that the hyphal of L.edodes could grow in all 4 culture,and formula 4(patato 200 g,sawdust 10 g,soybean powder 10 g,glucose 20 g,agar 20 g,water 1 L)was much more suitable for the growth of this strain,which was better than other culture conditions.And the growth rate of mycelium was the highest,reaching 0.51 cm/d,vigorous,white,and the colonies without pigment.Formula A(wood chips 78%,wheat bran 20%,lime 1%,gypsum 1%)was more suitable for long-term culture and preservation of mother spawn,and the germination rate of mycelium is higher,and its growth is vigorous and neat,and the colonies without pigment,may preserve 15 months.【Conclusion】Studies on selecting the cultures for purification and preservation of wild L.edodes,which laid a foundation for the collection,purification and long-term preservation of wild resources of L.edodes.

关 键 词：香菇 母种 培养基 纯化 保藏 

分 类 号：S646.12[农业科学—蔬菜学]