球孢白僵菌利用D-PPA合成D-HPPA的发酵条件优化  

Optimization of fermentation conditions for the synthesis of D-HPPA from D-PPA by Beauveria bassiana

作　　者：彭继兰 余杰[1] 刘宗求 王金华[1] 高娃 王永泽[1] PENG Ji-lan;YU Jie;LIU Zong-qiu;WANG Jin-hua;GAO Wa;WANG Yong-ze(Cooperative Innovation Center of Industrial Fermentation,Ministry of Education&Hubei Province/Key Laboratory of Fermentation Engineering,Ministry of Education/Hubei Key Laboratory of Industrial Microbiology,Hubei University of Technology,Wuhan 430068,China)

机构地区：[1]湖北工业大学教育部工业发酵省部共建协同创新中心/发酵工程教育部重点实验室/工业微生物湖北省重点实验室,武汉430068

出　　处：《湖北农业科学》2024年第9期95-101,共7页Hubei Agricultural Sciences

基　　金：国家自然科学基金青年项目(31501677);湖北工业大学博士启动基金项目(XJ2021010301);企业合作项目(2020240)。

摘　　要：为了提高球孢白僵菌(Beauveria bassiana)利用R-(+)-2-苯氧基丙酸(D-PPA)合成R-(+)-2-(4-羟基苯氧基)丙酸(D-HPPA)的能力,在单因素试验的基础上,通过Plackett-Burman设计和Box-Behnken中心组合设计优化球孢白僵菌生物合成D-HPPA的液态发酵条件。结果表明,当D-PPA初始添加浓度为40 g/L时,液态发酵最优条件为葡萄糖38.70 g/L、蚕蛹粉15 g/L、FeCl_(2)1.0 g/L、接种量15%(V/V)、pH 6.6及发酵温度28℃时,D-HPPA的产量为(25.10±0.43)g/L,D-HPPA的产率为57.24%±0.97%,比优化前提高了159%。In order to improve the ability of Beauveria bassiana to synthesize R-(+)-2-(4-hydroxyphenoxy)propionic acid(D-HP-PA)from R-(+)-2-phenoxy propionic acid(D-HPPA),on the basis of single factor test,the liquid fermentation conditions of D-HPPA biosynthesis by Beauveria bassiana were optimized by Plackett-Burman design and Box-Behnken center combination design.The results showed that when the initial concentration of D-PPA was 40 g/L,the optimal conditions for liquid fermentation were glu-cose 38.70 g/L,chrysalis powder 15 g/L,FeCl_(2)1.0 g/L,inoculation amount 15%(V/V),pH 6.6 and fermentation temperature 28℃.The yield of D-HPPA was(25.10±0.43)g/L,and the yield of D-HPPA was 57.24%±0.97%,which was 159%higher than that before optimization.

关 键 词：球孢白僵菌(Beauveria bassiana) 液态发酵 条件优化 R-(+)-2-(4-羟基苯氧基)丙酸 PLACKETT-BURMAN设计 Box-Behnken中心组合设计 

分 类 号：S482.4[农业科学—农药学]