α-细辛醚上调细胞色素C及Caspase-3表达影响食管癌Eca-109细胞的生物学行为  

The influence ofα-asarone in biological behavior of esophageal cancer Eca-109 cells by up-regulating expression of cytochrome C and Caspase-3

作　　者：黄静[1] 储韬 HUANG Jing;CHU Tao(Department of Pharmacology,Anhui Medical College,Anhui 230601)

机构地区：[1]安徽医学高等专科学校药学院,安徽230601

出　　处：《南通大学学报（医学版）》2024年第4期342-345,共4页Journal of Nantong University(Medical sciences)

基　　金：安徽省自然科学基金资助项目(2208085MH262)。

摘　　要：目的:分析α-细辛醚上调细胞色素C(cytochrome C,cytC)及Caspase-3表达对食管癌Eca-109细胞生物学行为的影响。方法:将食管癌Eca-109细胞随机等量分为4组,即低、中及高剂量(分别给予25、50及100 mg/L的α-细辛醚),另随机取等量细胞加入等体积培养液作为空白组,培养48 h后通过平板克隆实验检测4组细胞增殖情况,Annexin V/PI法检测细胞凋亡情况,RT-qPCR及Western Blot法检测细胞中cytC及Caspase-3 mRNA及蛋白表达。结果:(1)细胞克隆数在空白组最高,低剂量组显著高于高剂量组;凋亡率在空白组最低,低剂量组显著低于高剂量组。(2)4组细胞迁移至小室下的细胞数空白组>低剂量组>中剂量组>高剂量组。(3)CytC及Caspase-3蛋白在高剂量组表达显著高于空白组及低剂量组,且中剂量组表达高于空白组。(4)CytC及Caspase-3 mRNA的表达:高剂量组>中剂量组>低剂量组>空白组,差异均有统计学意义(均P<0.05)。结论:α-细辛醚抑制Eca-109细胞增殖及侵袭,并且可能通过上调cytC及Caspase-3表达促进调亡。Objective:To analyze influence ofα-asarone affects biological behavior of esophageal cancer Eca-109 cells by up-regulating expression of cytochrome C(cytC)and Caspase-3.Methods:Eca-109 cells of esophageal cancer were randomly divided into four groups,low dose,medium dose and high dose groups(administered 25,50 and 100 mg/L ofα-asarone,respectively),and the same amount of cells severed as the blank group withoutα-asarone.After 48 hours of culture,the cell proliferation of four groups were detected with plate cloning experiment and apoptosis of cells was detected with Annexin V/PI method,and cytC and Caspase-3 protein and mRNA in cells were detected by RT-qPCR and Western Blot.Results:(1)The number of cell clones was the highest in the blank group,and the low dose group was significantly higher than the high dose group.The apoptosis rate of the four groups was the lowest in the blank group,and the low dose group was significantly lower than the high dose group.(2)Four groups of cells migrated to the cell number blank group>low dose group>medium dose group>high dose group.(3)The expression of cytC and Caspase-3 protein in highdose group was significantly higher than that in blank group and low dose group,and the expression in medium dose group was higher than that in blank group.(4)The expression of cytC and Caspase-3 mRNA in each group was as follows:high dose group>medium dose group>low dose group>blank group(all P<0.05).Conclusion:α-asarone inhibits proliferation and invasion of Eca-109 cells and promotes apoptosis by up-regulating expression of cytC and Caspase-3.

关 键 词：Α-细辛醚 细胞色素C CASPASE-3 食管癌ECA-109细胞 增殖 凋亡 

分 类 号：R735.1[医药卫生—肿瘤]