牙周炎患者牙周膜组织miR-106a、miR-34a水平与炎症反应、牙槽骨吸收的相关性  

Correlation between the levels of miR-106a,miR-34a in periodontal ligament tissue and inflammatory response,alveolar bone resorption in patients with periodontitis

作　　者：沈丹 王林红[2] Shen Dan;Wang Linhong(Department of Stomatology,Xiaoshan Hospital Affiliated to Hangzhou Normal University,Hangzhou 311200,China;Department of Stomatology,Zhejiang Provincial People’s Hospital,Hangzhou 310000,China)

机构地区：[1]杭州师范大学附属萧山医院口腔科,浙江杭州311200 [2]浙江省人民医院口腔科,浙江杭州310000

出　　处：《温州医科大学学报》2024年第9期732-737,共6页Journal of Wenzhou Medical University

基　　金：浙江省医药卫生科技计划项目(2022KY568)。

摘　　要：目的:分析牙周炎患者牙周膜组织miR-106a、miR-34a水平与炎症反应、牙槽骨吸收的相关性。方法:选取2021年6月至2023年6月期间在杭州师范大学附属萧山医院接受治疗的81例牙周炎患者为牙周炎组,另选取在本院接受治疗的牙齿正畸患者81例作为对照组。入院后行牙周检查,包括菌斑指数(PLI)、龈沟出血指数(BI)、探诊深度(PD)、附着丧失(AL)。采用RT-PCR法测定牙周膜组织miR-106a、miR-34a的表达水平;采用ELISA测定热休克蛋白60(HSP60)、趋化因子12(CXCL12)、细胞间黏附分子-1(ICAM-1)、IL-17、I型胶原交联C-末端肽(CTX)、I型前胶原氨基末端肽(PINP)、骨保护素(OPG)、Runt相关转录因子2(Runx2)、I型胶原交联氨基末端肽(NTX)表达水平。Pearson法分析牙周膜组织miR-106a、miR-34a表达与炎症反应、牙槽骨吸收指标的相关性;ROC曲线分析牙周膜组织miR-106a、mi R-34a对牙周炎的诊断价值。结果:与对照组相比,牙周炎组患者PLI、BI、PD、AL表达水平显著升高(P<0.05)。与对照组相比,牙周炎组患者牙周膜组织中miR-106a表达水平降低,miR-34a表达水平显著增加(P<0.05)。与对照组相比,牙周炎组患者HSP60、CXCL12、ICAM-1、IL-17表达水平均显著升高(P<0.05),槽骨吸收指标中CTX、NTX表达水平显著升高,PINP、OPG、Runx2表达水平显著降低,差异均有统计学意义(P<0.05)。Pearson相关性分析显示,miR-106a与HSP60、CXCL12、ICAM-1、IL-17、CTX、NTX水平呈负相关(P<0.05),与PINP、OPG、Runx2水平呈正相关(P<0.05)。miR-34a表达水平与HSP60、CXCL12、ICAM-1、IL-17、CTX、NTX水平呈正负相关(P<0.05),与PINP、OPG、Runx2水平呈负正相关(P<0.05)。牙周膜组织miR-106a、miR-34a二者联合诊断牙周炎的AUC最高,优于牙周膜组织miR-106a、miR-34a各自单独诊断(Z_(二者联合-miR-106a)=2.553、P=0.011,Z_(二者联合-miR-34a)=2.834、P=0.005)。结论:牙周炎患者牙周膜组织miR-106a水平显著降低,miR-34a水平显�Objective:To analyze the correlation between the levels of miR-106a,miR-34a in periodontal ligament tissue and inflammatory response,alveolar bone resorption in patients with periodontitis.Methods:From June 2021 to June 2023,81 patients with periodontitis who received treatment in Xiaoshan Hospital Affiliated to Hangzhou Normal University were collected as the periodontitis group,another 81 orthodontic patients who received treatment in the same hospital were collected as the control group.Reverse transcription polymerase chain reaction(RT-PCR)method was applied to determine the expression levels of miR-106a and miR-34a in periodontal ligament tissue;Heat shock protein 60(HSP60),chemokine 12(CXCL12),intercellular adhesion molecule-1(ICAM-1),interleukin-17(IL-17),cross-linked C-terminal peptide of type I collagen(CTX),procollagen amino-terminal peptide of type I(PINP)and osteoprotegerin(OPD)were determined by enzyme-linked immunosorbent assay(ELISA).Pearson method was applied to analyze the correlation between the expression of miR-106a,miR-34a in periodontal ligament tissue,and inflammatory response,alveolar bone resorption indicators.The ROC curve was used to analyze the diagnostic value of miR-106a and miR-34a in periodontal ligament tissue.Results:Compared with the control group,the expression levels of plaque index(PLI),gingival sulcus bleeding index(BI),probing depth(PD)and attachment loss(AL)in periodontitis group were significantly higher(P<0.05).Compared with the control group,the expression levels of HSP60,CXCL12,ICAM-1 and IL-17 in periodontitis group were significantly increased(P<0.05),and the expression levels of CTX and NTX were significantly increased,while the expression levels of PINP,OPG and Runx2 were significantly decreased,with statistical significance(P<0.05).According to Pearson correlation analysis,miR-106a was negatively correlated with levels of HSP60,CXCL12,ICAM-1,IL-17,CTX,and NTX(P<0.05),and positively correlated with levels of PINP,OPG,and Runx2(P<0.05).The expression level of miR-34

关 键 词：牙周炎 miR-106a MIR-34A 炎症反应 牙槽骨吸收 

分 类 号：R73[医药卫生—肿瘤]