蜀山城市森林公园主要林分的土壤细菌群落特征及其影响因素  

Soil Bacterial Community Characteristics and Influencing Factors in the Major Forest Stands of Shushan Urban Forest Park

作　　者：王敉敉 崔珺[3] 滕臻 WANG Mimi;CUI Jun;TENG Zhen(College of Design and Architecture,Zhejiang Wanli University,Ningbo 315100,Zhejiang,China;School of Forestry Landscape Architecture,Anhui Agricultural University,Hefei 230036,Anhui,China;School of Life and Environmental Science,Huangshan University,Huangshan 245021,Anhui,China;School of Environmental Engineering,Xuzhou University of Technology,Xuzhou 221018,Jiangsu,China)

机构地区：[1]浙江万里学院设计艺术与建筑学院,浙江宁波315100 [2]安徽农业大学林学与园林学院,安徽合肥230036 [3]黄山学院生命与环境科学学院,安徽黄山245021 [4]徐州工程学院环境工程学院,江苏徐州221018

出　　处：《西北林学院学报》2024年第5期176-186,共11页Journal of Northwest Forestry University

基　　金：国家自然科学基金项目“不同林龄序列亚热带常绿阔叶林地下碳氮耦合循环特点”(31370626);安徽省高校省级自然科学研究重点项目“生态修复大背景下基于3S技术的合肥环巢湖湿地公园的景观格局变化研究”(KJ2021A0614);(24年国库基本科研)新引进高层次人才科研启动费项目(SC1032410380140)。

摘　　要：基于暖温带-亚热带过渡带且人为活动频繁的城市森林生态系统,从数量生态学角度出发,系统分析蜀山城市森林公园“植被-土壤-细菌”之间的相互作用机制。采用高通量测序技术,全面分析近郊城市森林绿地土壤细菌群落多样性,明确城市森林土壤细菌群落结构的主导影响因素。结果显示,与次生林相比,人工林普遍表现出较低的土壤pH值、含水量、有机碳和营养物质含量,表明人工造林和人类活动会加速土壤酸化。次生林土壤铵态氮(AN)、可溶性有机碳(DOC)含量与人工林差异性显著。人工林土壤细菌OTUs总数较低,马尾松林、麻栎林土壤细菌α多样性指数(S_1指数、H′指数)和次生林差异显著。酸杆菌门、变形杆菌门和放线菌门是主要菌群;次生林土壤酸杆菌门、变形杆菌门相对丰度高于人工林,放线菌门相对丰度低于马尾松林和麻栎林,绿弯菌门相对丰度低于人工林,麻栎林土壤芽单胞菌门相对丰度高于其他林分。土壤pH、AN和DOC是影响细菌群落分布模式的主要理化因子。本研究揭示了地上植被指标与地下土壤理化特性、细菌多样性指标的相关性。Based on the urban forest ecosystem in the transition zone between the warm temperate zone and subtropical zone with frequent human activities,taking"Shushan Urban Forest Park"as the research object,this study systematically analyzed the interaction mechanism between"vegetation-soil-bacteria"from the perspective of quantitative ecology.High-throughput sequencing technology was used to comprehensively analyze the diversity of soil bacterial communities in green spaces of suburb forests,and to clarify the dominant influencing factors of soil bacterial community structure in urban forests.The results showed that plantation forests generally exhibited lower soil pH,water content,organic carbon and nutrient content compared to secondary forests,suggesting that plantation forestry and human activities accelerate soil acidification.Significant differences were found in soil ammonium nitrogen(AN)and dissolved organic carbon(DOC)between secondary forests and planted forests.The total number of soil bacterial OTUs was lower in plantation forests,and the alpha diversity indices(S1 index,H'index)of the soils of Pinus massoniana and Quercus acutissima forests were significantly different from those of secondary forests.The dominant bacterial phyla were Acidobacteria,Proteobacteria,and Actinobacteria,with higher relative abundance of Acidobacteria and Proteobacteria in secondary forests compared to plantation forests,and lower relative abundance of Actinobacteria compared to P.massoniana forests and Q.acutissima forests.The relative abundance of Chloroflexi phylum was lower in secondary forests compared to plantation forests,whereas the relative abundance of Gemmatimonadetes was higher in Q.acutissima forests compared to other forests.Soil pH,AN and DOC were identified as the main physicochemical factors influencing the distribution pattern of bacterial communities.This study reveals the correlation between above-ground vegetation indicators and below-ground soil physicochemical characteristics and bacterial diversity indicato

关 键 词：城市森林 人工林 次生林 土壤理化性质 细菌群落特征 

分 类 号：S154.36[农业科学—土壤学]