基于超高效液相色谱-四极杆-静电场轨道阱质谱技术与网络药理学揭示金银花与忍冬叶化学成分差异及抗衰老机制  

Analysis of Differences in Chemical Components between Ethanol Extracts from Flower Buds and Leaves of Honeysuckle(Lonicera japonica)and Their Anti-aging Mechanisms Using Ultra-high Performance Liquid Chromatography-Quadrupole Electrostatic Field Orbitrap Mass Spectrometry and Network Pharmacology

作　　者：刘舒鹏 黄厚钰 王笑雪 郝巨辉 李卫东[1,2] LIU Shupeng;HUANG Houyu;WANG Xiaoxue;HAO Juhui;LI Weidong(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Collaborative Innovation Center of Aerospace and Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China;Beijing Tongrentang Supply Chain Management Co.Ltd.,Beijing 100011,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]北京中医药大学航空航天中医药协同创新中心,北京102488 [3]北京同仁堂供应链管理有限责任公司,北京100011

出　　处：《食品科学》2024年第20期1-11,共11页Food Science

基　　金：国家自然科学基金面上项目(31370360)。

摘　　要：目的:探讨金银花醇提物(ethanol extract of Lonicera japonica flos,EELF)与忍冬叶醇提物(ethanol extract of L.japonica leaves,EELL)化学成分差异、抗衰老潜在活性成分及机制。方法:采用超高效液相色谱-四极杆-静电场轨道阱质谱(ultra-high performance liquid chromatography-quadrupole electrostatic field orbitrap mass spectrometry,UPLC-QE-Orbitrap-MS)测定EELF和EELL,解析两者间化学成分差异。采用含不同质量浓度EELF和EELL的Escherichia coli OP50菌液喂养秀丽隐杆线虫,考察线虫寿命、运动能力、生殖能力、抗氧化应激能力等指标变化。通过网络药理学探究其抗衰老的潜在作用机制。结果:EELF和EELL中共鉴定出122种化合物,有机酸类化合物35种、黄酮类化合物27种、环烯醚萜类化合物37种、其他化合物23种。EELF和EELL化学成分组成大致相同,绿原酸、总酚酸等含量也大致相同,EELL中总黄酮、异绿原酸C和木犀草苷含量显著高于EELF,而异绿原酸A含量显著低于EELF。不同质量浓度的EELF和EELL均能提高线虫抗氧化应激及热应激能力、延长线虫的寿命、提高线虫运动与生殖能力,并降低活性氧与脂褐素水平。共筛选出EELF和EELL中16个潜在活性成分和抗衰老潜在靶点59个,核心靶点有信号转导和转录激活因子3、丝氨酸/苏氨酸激酶1、表皮生长因子受体、类固醇受体辅激活因子、胱天蛋白酶3、肿瘤坏死因子等;主要通过癌症通路、磷脂酰肌醇-3-激酶/蛋白激酶B信号通路、血脂与动脉粥样硬化等通路发挥抗衰老作用。结论:EELF和EELL具有相似的化学成分,发挥着相似的抗衰老作用,具有多途径、多靶点的特性,本研究可为其深度开发利用提供参考。Objective:To investigate the differences in chemical composition between ethanol extracts from Lonicera japonica flos(EELF)and L.japonica leaves(EELL)and their potential anti-aging components and mechanisms.Methods:The chemical compositions of EELF and EELL were determined by ultra-high performance liquid chromatography-quadrupole electrostatic field orbitrap mass spectrometry(UPLC-QE-Orbitrap-MS).The effect of feeding Escherichia coli OP50 culture containing different concentrations of EELF or EELL on the life span,mobility,reproductive capacity and oxidant stress resistance of Caenorhabditis elegans was investigated.The potential anti-aging mechanism of EELF and EELL was explored through network pharmacology.Results:A total of 122 compounds were identified in EELF and EELL,including 35 organic acids,27 flavonoids,37 iridoid compounds and 23 other compounds.The chemical compositions of EELF and EELL were roughly the same,and the contents of chlorogenic acid and total phenolic acid were also roughly same.The contents of total flavonoids,isochlorogenic acid C and luteolin in EELL were significantly higher than those in EELF,while the content of isochlorogenic acid A was significantly lower than that in EELF.EELF and EELL at all concentrations improved oxidative and thermal stress resistance,prolonged the life span,enhanced the motility and reproductive capacity of the nematode,and decreased the levels of reactive oxygen species(ROS)and lipofuscin.A total of 16 potential bioactive components from EELF and EELL and 59 potential anti-aging targets for them were identified,and the core targets were signal transducer and activator of transcription 3(STAT3),serine/threonine kinase 1(AKT1),epidermal growth factor receptor(EGFR),steroid receptor coactivator(SRC),cysteinyl aspartate specific proteinase 3(CASP3),and tumor necrosis factor(TNF).The antiaging effect could be exerted by suppressing cancer,reducing lipid and alleviating atherosclerosis through the regulation of the phosphatidyl-inositol 3-kinase/serine-threonine

关 键 词：金银花 忍冬叶 化学成分 抗衰老 网络药理学 

分 类 号：R932[医药卫生—生药学]