PSGL-1与皮肤恶性黑色素瘤预后的相关性分析  

Correlation analysis between PSGL-1 and cutaneous malignant melanoma

作　　者：刘梓燊 郑瑛瑛 袁梦琪 张甘霖[1] 杨国旺[1] LIU Zishen;ZHENG Yingying;YUAN Mengqi;ZHANG Ganlin;YANG Guowang(Department of Oncology,Beijing Hospital of Traditional Chinese Medicine,Capital Medical University,Beijing 100010,China)

机构地区：[1]首都医科大学附属北京中医医院肿瘤科,北京100010

出　　处：《中山大学学报（自然科学版）（中英文）》2024年第5期83-89,共7页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：国家自然科学基金(82174453,82374538);北京市自然科学基金(7222270)。

摘　　要：为了探究PSGL-1与皮肤恶性黑色素瘤预后是否具有相关性,从全基因组关联研究(GWAS)提取PSGL-1和皮肤恶性黑色素瘤的遗传数据,使用逆方差加权法、MR-Egger回归法、加权中位数法、加权模式法和简单模式法进行分析,并对数据进行敏感性分析。从TCGA数据库提取皮肤恶性黑色素瘤的生存信息,根据PSGL-1表达高低进行分组,通过Kaplan-Meier法绘制生存曲线,Log-rank检验比较两者生存期之间的差异。通过体外实验探究PSGL-1对A375细胞生物学功能影响。结果显示PSGL-1对皮肤恶性黑色素瘤的逆方差加权法分析结果为OR=0.666,95%CI(0.494~0.899),P=0.008。PSGL-1高表达组中位生存期为44.70个月,PSGL-1低表达组中位生存期为29.13个月,P=0.0002。PSGL-1体外抑制A375细胞的迁移。研究结果发现PSGL-1表达增高是皮肤恶性黑色素瘤的保护因素,是一种潜在的皮肤黑色素瘤预后标志物。To investigate whether PSGL-1 is correlated with the prognosis of cutaneous malignant melanoma.Genetic data of PSGL-1 and cutaneous malignant melanoma were extracted from genomewide association studies(GWAS)and analyzed using inverse variance weighting,MR-Egger regres-sion,weighted median,weighted mode,and simple mode methods,and sensitivity analyses were performed on the data.Survival information of cutaneous malignant melanoma was extracted from the TCGA database,and grouped according to high and low PSGL-1 expression,survival curves were plotted by the Kaplan-Meier method,and the Log-rank test was used to compare the difference between the two survival periods.Exploring the effects of PSGL-1 on the biological functions of A375 cells through in vitro experiments.The inverse variance weighted method analysis of PSGL-1 on cuta-neous malignant melanoma resulted in OR=0.666,95%CI(0.494-0.899),P=0.008.The median survival was 44.70 months in the PSGL-1 high expression group and 29.13 months in the PSGL-1 low expression group,P=0.0002.PSGL-1 inhibits the migration of A375 cells in vitro.Increased PSGL-1 expression is a protective factor against cutaneous malignant melanoma and is a potential prognostic marker for cutaneous melanoma.

关 键 词：PSGL-1 皮肤恶性黑色素瘤 孟德尔随机化 TCGA数据库 

分 类 号：R73[医药卫生—肿瘤]