HPLC-UV法同时测定赤血康脉胶囊中五种成分  

The HPLC-UV Method Simultaneously Determined the Five Components in Chixue Kangmai Capsules

作　　者：吴永刚[1] 赵譞 蔡宇宇 孙海珍 白天 张晓燕[1] WU Yonggang;ZHAO Xuan;CAI Yuyu;SUN Haizhen;BAI Tian;ZHANG Xiaoyan(Heilongjiang University of Chinese Medicine,Harbin 150040,China;Branch Campus of Daqing,Harbin Medical University,Daqing 163311,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040 [2]哈尔滨医科大学大庆校区,黑龙江大庆163311

出　　处：《特产研究》2024年第5期95-100,共6页Special Wild Economic Animal and Plant Research

基　　金：国家自然科学基金项目(82274238)。

摘　　要：本研究旨在建立HPLC-UV法同时测定赤血康脉胶囊中芍药苷、毛蕊异黄酮葡萄糖苷、β-蜕皮甾酮、阿魏酸和党参炔苷的含量。采用DiamonsilR C_(18)(250 mm×4.6 mm,5μm)色谱柱;流动相为乙腈-0.1%磷酸(梯度洗脱);流速为1.0 mL/min;柱温为35℃;检测波长为268 nm,进行分析。结果显示,5种化学成分在各自范围内线性关系良好(r>0.9995);精密度、稳定性、重复性试验结果的RSD均小于2.0%,平均加样回收率99.35%~101.59%,RSD 0.95%~2.69%。该含量测定方法简单可靠、重复性好,操作便捷,适用于同时测定赤血康脉胶囊中五种成分的含量。The purpose of this study was to establish an HPLC-UV method to simultaneously determine the contents of paeoniflorin,calycosin-7-glucoside,-ecdysterone,ferulic acid and lobetyolin in ChixueKangmai capsules.The chromatographic separation of HPLCmethod was achieved on a C_(18)column(250 mm×4.6 mm,5μm)with acetonitrile-0.1%phosphoric acid solution as mobile phase at the flow rate of 1.0 mLmin^(-1) for gradient elution.The detection wavelength was 268 nmand the column temperature was 35℃for analysis.The results showed that the five chemical components showed good linear relationships within their respective ranges(r>0.9995),the RSD of precision,stability and repeatability were less than 2.0%,and the average sample recovery rate of 99.35%to 101.59%and an RSDof 0.95%to 2.69%.The methed is ideal for simultaneous assessment of the contents of five components in the ChixueKangmai capsules and is straightforward,dependable,reproducible,and simple to use.

关 键 词：赤血康脉胶囊 HPLC-UV 含量测定 

分 类 号：R284.1[医药卫生—中药学]