长春花内生真菌Aspergillus sp.CCH-1E的活性次级代谢产物研究  

Bioactive secondary metabolites from an endophytic fungus Aspergillus sp.CCH-1E from Catharanthus roseus

作　　者：王雅[1] 陈育伍 刘博 张璇 康雅男 蓝伟 王子墨 孙奕[2] WANG Ya;CHEN Yu-wu;LIU Bo;ZHANG Xuan;KANG Ya-nan;LAN Wei;WANG Zi-mo;SUN Yi(School of Life Science and Engineering,Lanhzou University of Technology,Lanzhou 730050,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国中药杂志》2024年第17期4687-4694,共8页China Journal of Chinese Materia Medica

基　　金：中国中医科学院科技创新工程项目(CI2021A04514)。

摘　　要：该文对长春花内生真菌Aspergillus sp.CCH-1E的次级代谢产物进行研究。采用正反相柱色谱、高效液相色谱等技术从菌株Aspergillus sp.CCH-1E的大米发酵物中分离得到12个化合物,运用波谱学(UV、IR、NMR、HR-ESI-MS等)方法进行数据分析和文献比对,鉴定了化合物结构,分别为chermesinone H(1)、chermesinone I(2)、chermesinone B(3)、8,11-didehydrochermesinone B(4)、chermesinone C(5)、chermesinone A(6)、chevalone B(7)、barbacenic acid(8)、3,6,8-三羟基-3,5,7-三甲基-3,4-二氢异香豆素(9)、5-羟基-2-甲氧基-7-甲基-1,4-萘醌(10)、1-羟基-6,8-二甲氧基-3-甲基蒽-9,10-二酮(11)、7-drimen-9α,11,12-triol(12)。其中,化合物1和2为azaphilone类新化合物。采用MTT法测定了所有化合物对人非小细胞肺癌细胞A549、NCI-H1650及人宫颈癌细胞HeLa的生长抑制作用。结果显示,化合物7可显著地抑制3种细胞生长,IC_(50)为1.22~2.43μmol·L^(-1);化合物1~6也表现出中等的细胞生长抑制作用(IC50为16.24~35.28μmol·L^(-1))。This study focused on the bioactive secondary metabolites of an endophytic fungus Aspergillus sp.CCH-1E from Catharanthus roseus.The secondary metabolites from Aspergillus sp.CCH-1E were isolated by using various chromatographic methods[such as normal-phase and reversed-phase chromatography and high-performance liquid chromatography(HPLC)],and their structures were identified by various spectroscopic methods[e.g.,ultraviolet(UV)spectroscopy,infrared(IR)spectroscopy,nuclear magnetic resonance(NMR)spectroscopy,and high-resolution electrospray ionization mass spectrometry(HR-ESI-MS)].Twelve compounds were yielded and identified from Aspergillus sp.CCH-1E,which are chermesinone H(1),chermesinone I(2),chermesinone B(3),8,11-didehydrochermesinone B(4),chermesinone C(5),chermesinone A(6),chevalone B(7),barbacenic acid(8),3,6,8-trihydroxy-3,5,7-trimethyl-3,4-dihydroisocoumarin(9),5-hydroxy-2-methoxy-7-methyl-1,4-naphthoquinone(10),1-hydroxy-6,8-dimethoxy-3-methylanthracene-9,10-dione(11),and 7-drimen-9α,11,12-triol(12).Among them,compounds 1 and 2 are new compounds.The growth inhibition effects of all compounds were evaluated against non-small cell lung cancer cell lines A549 and NCIH1650,as well as human cervical cancer cell line HeLa by using methylthiazolyldiphenyl-tetrazolium bromide(MTT).Compound 7 significantly inhibited the growth of three tumor cells with the IC_(50)values of 1.22-2.43μmol·L^(-1),respectively.Compounds 1-6 showed moderate cell growth inhibition with the IC50 values of 16.24-35.28μmol·L^(-1).

关 键 词：长春花 内生真菌 曲霉属 次级代谢产物 细胞生长抑制 

分 类 号：R284.1[医药卫生—中药学] TQ28[医药卫生—中医学]