Inter-chromosomal insertions into wild-type chromosomes induced by SCRaMbLE  

作　　者：Sijie Zhou Junyanrui Li Xichen Cui Ying Wang Ying-Jin Yuan 

机构地区：[1]Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin 300072,China [2]Frontiers Research Institute for Synthetic Biology,Tianjin University,Tianjin 300072,China

出　　处：《Frontiers of Chemical Science and Engineering》2024年第9期125-135,共11页化学科学与工程前沿（英文版）

基　　金：supported by the National Key Research and Development Program of China(Grant No.2021YFC2100800);the National Natural Science Foundation of China(Grant No.22208241);China Postdoctoral Science Foundation(Grant No.2023M732591);the Key R&D Program of Shandong Province,China(Grant No.2022SFGC0102).

摘　　要：Genomic rearrangements play a crucial role in shaping biological phenotypic diversity and driving species evolution.Synthetic chromosome rearrangement and modification by LoxP-mediated evolution(SCRaMbLE)has been applied to explore large-scale genomic rearrangements,yet it has been observed that these rearrangements occur exclusively in genomic regions containing loxPsym sites.Here,we found that SCRaMbLE of synthetic yeast harboring synthetic chromosome V and X can generate a variety of synthetic segment insertions into wild-type chromosomes,ranging from 1 to 300 kb.Furthermore,it was revealed that the novel insertions impacted the transcriptional level of neighboring regions and affected the production of exemplar pathway of zeaxanthin.Collectively,our results improve the understanding of the ability of SCRaMbLE to generate complex structural variations in nonsynthetic regions and provide a potential model to explore genomic transposable events.

关 键 词：genomic rearrangements synthetic yeast genome SCRAMBLE inter-chromosomal insertions synthetic biology 

分 类 号：Q78[生物学—分子生物学]