厚朴酚对牙周炎大鼠牙周组织中IL-6、VEGF表达的影响  

Effects of magnolol on the expression of IL-6 and VEGF in periodontal tissues of rats with periodontitis

作　　者：于丽贤 李春年[1] YU Lixian;LI Chunnian(School of Stomatology of Hebei Medical University-Stomatology Hospital/Hebei Key Laboratory of Stomatology,Shijiazhuang 050017,Hebei,China)

机构地区：[1]河北医科大学口腔医学院·口腔医院/河北省口腔医学重点实验室,河北石家庄050017

出　　处：《现代中西医结合杂志》2024年第15期2062-2065,2077,共5页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河北省中医药管理局科研计划项目(2014135)。

摘　　要：目的观察厚朴酚对牙周炎大鼠牙周病变及牙周组织中白细胞介素-6(IL-6)、血管内皮生长因子(VEGF)表达的影响,探讨厚朴酚治疗牙周炎的价值。方法取3月龄雄性SD大鼠65只,随机选取其中10只作为对照组,其余大鼠采用正畸结扎丝结扎双侧上颌第一磨牙方法建立牙周炎模型。结扎4周后,将造模成功的40只大鼠随机分为4组,每组10只。局部治疗组去除局部刺激因素即结扎丝;厚朴酚治疗组在不去除结扎丝的情况下给予厚朴酚50 mg/(kg·d)灌胃,局部+厚朴酚治疗组在去除结扎丝的同时给予厚朴酚50 mg/(kg·d)灌胃,对照组给予等剂量生理盐水灌胃,各组均干预8周。观察各组大鼠牙体牙周组织一般情况,HE染色观察牙周组织病理形态,免疫组化染色检测牙周组织中IL-6、VEGF阳性表达情况。结果牙周炎组大鼠双侧上颌第一磨牙龈缘红肿;结合上皮离开釉牙骨质界向根方移位形成深牙周袋,周围有大量炎细胞浸润,牙槽嵴顶及固有牙槽骨吸收破坏。局部治疗组双侧上颌第一磨牙龈红肿,探诊出血;牙周袋深,周围有少量炎细胞浸润,牙槽嵴顶及固有牙槽骨破坏严重,牙槽嵴顶可见新生骨形成。厚朴酚治疗组和局部+厚朴酚治疗组双侧上颌第一磨牙龈红肿较轻或无红肿;牙周袋浅,周围有少量炎性细胞浸润,牙槽嵴顶均可见新生骨形成,其中局部+厚朴酚治疗组牙周组织破坏更轻。牙周炎组大鼠牙周组织中IL-6及VEGF阳性表达平均光密度值均明显高于对照组(P均<0.05),厚朴酚治疗组和局部+厚朴酚治疗组大鼠牙周组织中IL-6及VEGF阳性表达平均光密度值均明显低于牙周炎组(P均<0.05),且局部+厚朴酚治疗组均明显低于局部治疗组(P均<0.05)。结论厚朴酚能够显著降低牙周炎大鼠牙周组织中的致炎细胞因子水平,减轻牙周病变,改善牙槽骨的新生状态。Objective It is to observe the effects of magnolol on periodontal lesions and the expressions of interleukin-6(IL-6)and vascular endothelial growth factor(VEGF)in periodontal tissues of periodontitis rats,and to explore the value of magnolol in the treatment of periodontitis.Methods Sixty-five 3-month-old male SD rats were selected,in which 10 rats were randomly selected as the control group,and the remaining rats were used to establish periodontitis models by ligating bilateral maxillary first molar teeth with orthodontic ligature wire.After 4 weeks of ligation,40 successfully modeled rats were randomly divided into 4 groups,with 10 rats in each group.Topical irritant ligature wire was removed in the topical treatment group;the magnolol treatment group was given 50 mg/(kg·d)of magnolol by gavage without removing the ligature wire,the topical+magnolol treatment group was given 50 mg/(kg·d)of magnolol by gavage based on removing the ligature wire,and the control group was given an equal volume of saline by gavage,all the groups were treated for 8 weeks.The general condition of the periodontal tissues and dentition of the rats in each group were observed,the pathological morphology of the periodontal tissues was observed by HE staining,and the positive expressions of IL-6 and VEGF in the periodontal tissues were detected by immunohistochemical staining.Results The bilateral maxillary first molar gingiva of rats in the periodontitis group were red and swollen;the combined epithelium left the enamel-osseous border and shifted toward the root to form deep periodontal pockets surrounded by a large number of inflammatory cells infiltrated,with resorption and destruction of the tops of alveolar ridges and intrinsic alveolar bone.The bilateral maxillary first molar gingiva of rats in the topical treatment group was red and swollen,with probing bleeding;the periodontal pockets were deep,with a small amount of inflammatory cell infiltration around them,and there was severe destruction of the alveolar ridge apex and intrin

关 键 词：牙周炎 厚朴酚 大鼠 白细胞介素-6 血管内皮生长因子 

分 类 号：R-332[医药卫生]